Download full-text PDF |
Source |
|---|
Publication Analysis
Top Keywords
Similar Publications
Broad substrate scope C-C oxidation in cyclodipeptides catalysed by a flavin-dependent filament.
Nat Commun
January 2025
University of St Andrews, School of Biology, North Haugh, Biomolecular Sciences Building, St Andrews, UK.
Cyclic dipeptides are produced by organisms across all domains of life, with many exhibiting anticancer and antimicrobial properties. Oxidations are often key to their biological activities, particularly C-C bond oxidation catalysed by tailoring enzymes including cyclodipeptide oxidases. These flavin-dependent enzymes are underexplored due to their intricate three-dimensional arrangement involving multiple copies of two distinct small subunits, and mechanistic details underlying substrate selection and catalysis are lacking.
View Article and Find Full Text PDFConstruction of antibiotic-free riboflavin producer in by metabolic engineering strategies with a plasmid stabilization system.
Synth Syst Biotechnol
June 2025
Key Laboratory of Industrial Biotechnology, Ministry of Education, School of Biotechnology, Jiangnan University, Wuxi, Jiangsu 214122, China.
Riboflavin, an important vitamin utilized in pharmaceutical products and as a feed additive, is mainly produced by metabolically engineered bacterial fermentation. However, the reliance on antibiotics in the production process leads to increased costs and safety risks. To address these challenges, an antibiotic-free riboflavin producer was constructed using metabolic engineering approaches coupled with a novel plasmid stabilization system.
View Article and Find Full Text PDFUnlocking the catalytic precision of ligand-controlled enzymatic halogenation.
Proc Natl Acad Sci U S A
January 2025
School of Biomolecular Science and Engineering, Vidyasirimedhi Institute of Science and Technology (VISTEC), Rayong 10120, Thailand.
A single-component flavin-dependent halogenase, AetF, has emerged as an attractive biocatalyst for catalyzing halogenation. However, its flavin chemistry remains unexplored and cannot be predicted due to its uniqueness in sequence and structure compared to other flavin-dependent monooxygenases. Here, we investigated the flavin reactions of AetF using transient kinetics.
View Article and Find Full Text PDFMolecular characterization of an endophytic strain of Bacillus subtilis with plant growth-promoting properties from a wild relative of papaya.
J Appl Microbiol
January 2025
ICAR-Indian Institute of Horticultural Research, Hessaraghatta Lake Post, Bengaluru 560089, Karnataka, India.
Aim: Bacillus subtilis is usually found in soil, and their biocontrol and plant growth-promoting capabilities are being explored more recently than ever. However, knowledge about metabolite production and genome composition of endophytic B. subtilis from seeds is limited.
View Article and Find Full Text PDFCRISPR/Cas13X-assisted programmable and multiplexed translation regulation for controlled biosynthesis.
Nucleic Acids Res
January 2025
Key Laboratory of Carbohydrate Chemistry and Biotechnology, Ministry of Education, Jiangnan University, No. 1800, Lihu Avenue, Binhu District, Wuxi 214122, China.
Developing efficient gene regulation tools is essential for optimizing microbial cell factories, but most existing tools only modulate gene expression at the transcriptional level. Regulation at the translational level provides a faster dynamic response, whereas developing a programmable, efficient and multiplexed translational regulation tool remains a challenge. Here, we have developed CRISPRi and CRISPRa systems based on hfCas13X that can regulate gene translation in Bacillus subtilis.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!