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Collaborative performance of enzymatic saccharification and organic pollutant degradation from PHP (phosphoric acid coupled with hydrogen peroxide) pretreatment of lignocellulose.
J Environ Manage
January 2025
College of Environmental Science, Sichuan Agricultural University, Chengdu, Sichuan, 611130, PR China; Sichuan Provincial Engineering Research Center of Agricultural Non-point Source Pollution Control, Sichuan Agricultural University, Chengdu, Sichuan, 611130, PR China.
As a newly developed technology, lignocellulose pretreatment of PHP (phosphoric acid coupled with hydrogen peroxide) can facilitate the enzymatic hydrolysis of pretreated lignocellulose for glucose production. It also has been found that the derived oxidative tail gas from pretreatment can facilely degrade organic pollutant. To balance the pollutant degradation and the glucose yield, the collaborative optimization on pretreatment was investigated.
View Article and Find Full Text PDFAdvances in fungal sugar transporters: unlocking the potential of second-generation bioethanol production.
Appl Microbiol Biotechnol
January 2025
Department of Biochemistry and Immunology, Ribeirão Preto Medical School, University of São Paulo, Ribeirão Preto, SP, 14049-900, Brazil.
Second-generation (2G) bioethanol production, derived from lignocellulosic biomass, has emerged as a sustainable alternative to fossil fuels by addressing growing energy demands and environmental concerns. Fungal sugar transporters (STs) play a critical role in this process, enabling the uptake of monosaccharides such as glucose and xylose, which are released during the enzymatic hydrolysis of biomass. This mini-review explores recent advances in the structural and functional characterization of STs in filamentous fungi and yeasts, highlighting their roles in processes such as cellulase induction, carbon catabolite repression, and sugar signaling pathways.
View Article and Find Full Text PDFSelf-sufficient biocatalytic cascade for the continuous synthesis of danshensu in flow.
Appl Microbiol Biotechnol
January 2025
Department of Chemistry, Biochemistry and Pharmaceutical Sciences, University of Bern, Freiestrasse 3, 3012, Bern, Switzerland.
A new strategy has been developed to successfully produce the active component danshensu ex vivo. For this purpose, phenylalanine dehydrogenase from Bacillus sphaericus was combined with the novel hydroxyphenylpyruvate reductase from Mentha x piperita, thereby providing an in situ cofactor regeneration throughout the conversion process. The purified enzymes were co-immobilized and subsequently employed in batch biotransformation, resulting in 60% conversion of 10 mM L-dopa within 24 h, with a catalytic amount of NAD as cofactor.
View Article and Find Full Text PDFIdentification of (PDZ-RhoGEF) as an in vivo regulator of synapses and cognition.
Proc Natl Acad Sci U S A
January 2025
Department of Comparative Biosciences, University of Wisconsin-Madison, Madison, WI 53706.
Given the influence of cognitive abilities on life outcomes, there is inherent value in identifying genes involved in controlling learning and memory. Further, cognitive dysfunction is a core feature of many neuropsychiatric disorders. Here, we use a combinatory in silico approach to identify human gene targets that will have an especially high likelihood of individually and directly impacting cognition.
View Article and Find Full Text PDFRapid detection of hydrogen peroxide and nitrite in adulterated cow milk using enzymatic and nonenzymatic methods on a reusable platform.
RSC Adv
January 2025
Environmental Biotechnology Laboratory, Department of Biological Sciences, Birla Institute of Technology and Science Pilani, Hyderabad Campus, Jawahar Nagar, Kapra Mandal, Medchal District Hyderabad 500078 India
Cow milk is readily adulterated due to its complex properties that can emulsify many adulterants. Among the commonly used adulterants in cow milk are hydrogen peroxide (HP) and nitrite. Commercially available HP is added to extend cow milk's shelf life, while nitrite enters through the tap or pond water added to increase cow milk's volume.
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