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Analysis of mechanisms of the rabies virus P protein-nucleocapsid interaction using engineered N-protein peptides and potential applications in antivirals design.
Antiviral Res
December 2024
Department of Biochemistry and Pharmacology, University of Melbourne, 3010, Parkville, VIC, Australia; Bio21 Molecular Science and Biotechnology Institute, University of Melbourne, 3010, Parkville, VIC, Australia. Electronic address:
The Phosphoprotein (P protein) of the rabies virus has multiple roles in virus replication. A critical function is to act as a cofactor in genome replication and mRNA production through binding via its N-terminal region to the L protein, the essential enzyme for mRNA and genome synthesis/processing, and via its C-terminal domain (P) to the N protein and viral RNA (N-RNA) ribonucleoprotein complex. The binding site of the P on the N protein is a disordered loop that is expected to be phosphorylated at Ser389.
View Article and Find Full Text PDFDimerization of Rabies Virus Phosphoprotein and Phosphorylation of Its Nucleoprotein Enhance Their Binding Affinity.
Viruses
November 2024
Institut de Biologie Structurale, Université Grenoble Alpes, CEA, CNRS, 38000 Grenoble, France.
The dynamic interplay between a multimeric phosphoprotein (P) and polymeric nucleoprotein (N) in complex with the viral RNA is at the heart of the functioning of the RNA-synthesizing machine of negative-sense RNA viruses of the order . P multimerization and N phosphorylation are often cited as key factors in regulating these interactions, but a detailed understanding of the molecular mechanisms is not yet available. Working with recombinant rabies virus (RABV) N and P proteins and using mainly surface plasmon resonance, we measured the binding interactions of full-length P dimers and of two monomeric fragments of either circular or linear N-RNA complexes, and we analyzed the equilibrium binding isotherms using different models.
View Article and Find Full Text PDFA label-free optical biosensor-based point-of-care test for the rapid detection of Monkeypox virus.
Biosens Bioelectron
February 2025
Department of Medicine, Division of Infectious Diseases and Global Public Health, University of California, San Diego, CA, 92093, USA. Electronic address:
Diagnostic approaches that combine the high sensitivity and specificity of laboratory-based digital detection with the ease of use and affordability of point-of-care (POC) technologies could revolutionize disease diagnostics. This is especially true in infectious disease diagnostics, where rapid and accurate pathogen detection is critical to curbing the spread of disease. We have pioneered an innovative label-free digital detection platform that utilizes Interferometric Reflectance Imaging Sensor (IRIS) technology.
View Article and Find Full Text PDFCats: The New Challenge for Rabies Control in the State of Yucatan, Mexico.
Pathogens
October 2024
Instituto de Diagnóstico y Referencia Epidemiológicos, Dirección General de Epidemiología, Secretaría de Salud, Francisco de P. Miranda 177, Colonia Unidad Lomas de Plateros, Alcaldía Álvaro Obregón C.P. 01480, Ciudad de México, Mexico.
Article Synopsis
- The increasing population in Yucatan is driving construction in the Mayan jungle for tourism, housing, and agriculture, alongside a rise in rabies cases in cats.
- A study was conducted to analyze rabies viruses in felines through genetic and antigenic characterization, using ArcGIS and R software for mapping case locations.
- Nine rabies cases from 2003-2022 were identified, revealing three antigenic variants predominantly linked to urban areas, emphasizing the risk of reintroducing rabies in dogs if vaccination rates decline.
Analysis of the antigenic and immunogenic properties of the native rabies virus glycoprotein purified by Lens culinaris lectin affinity chromatography.
J Virol Methods
January 2025
Instituto Pasteur, São Paulo, Brazil. Electronic address:
Rabies virus glycoprotein (RABV-G) is responsible for the recognition of specific cell surface receptors and induces the production of neutralizing antibodies (VNA). Since RABV-G is a glycoprotein, this work aimed to evaluate Lens culinaris (LCA) chromatography as a simple and effective purification method. The purity and identification of the protein obtained were analyzed by SDS-PAGE, ELISA and lectin-binding assay.
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