AI Article Synopsis
- The study amplified a specific gene responsible for antibiotic resistance in Enterococcus faecalis, Enterococcus faecium, and a strain of Streptococcus agalactiae using PCR.
- The findings showed that the antibiotic resistance genes in these species are very similar or homologous.
- Dot blot hybridization assays confirmed the presence of two related gene segments in all the tested isolates that exhibited high-level resistance to gentamicin.
Article Abstract
The structural gene coding for the bifunctional aminoglycoside-modifying 6'-acetyltransferase-2''-phosphotransferase (6'AAC-2''APH) enzyme was specifically amplified by the polymerase chain reaction using template DNA of clinical isolates of enterococci (both Enterococcus faecalis and Enterococcus faecium) and the single high-level gentamicin-resistant Streptococcus agalactiae strain identified thus far. The results of the present study demonstrated that the genes encoding this antibiotic resistance trait are highly homologous in these species. In dot blot hybridization assays using nonradioactively labeled oligonucleotide probes, strains with and without high-level gentamicin resistance could be discerned unequivocally. The gene segment encoding 6'-acetylating activity and the gene segment encoding 2''-phosphorylating activity were simultaneously present in all isolates exhibiting high-level gentamicin resistance.
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| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC190320 | PMC |
| http://dx.doi.org/10.1128/AAC.36.6.1215 | DOI Listing |
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