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[Construction of a recombinant strain expressing SpaA and CbpB of and evaluation of the strain immunogenicity in a mouse model].
Sheng Wu Gong Cheng Xue Bao
December 2024
College of Veterinary Medicine, Southwest University, Chongqing 402460, China.
To construct a recombinant strain expressing SpaA and CbpB of for oral administration, we constructed the recombinant plasmid pDG1730-CBJA by fusion PCR and seamless cloning. The plasmid was introduced into . KC strain by natural transformation, and the recombinant strain KC-- was screened out on the plate containing spectinomycin () and confirmed by PCR and starch degradation test.
View Article and Find Full Text PDFA field comparison study of two vaccine protocols against Erysipelothrix rhusiopathiae in two types of swine breeds in Spain.
BMC Vet Res
October 2024
Department of Community Nursing, Preventive Medicine and Public Health and History of Science, University of Alicante, Campus de Sant Vicent del Raspeig. Ap. 99, Alicante, E-03080, Spain.
Article Synopsis
- Erysipelas continues to significantly impact the pig industry economically, and preventing it in young pigs relies heavily on maternal immunity, which can be enhanced through strategic vaccination.
- A study investigated the effects of two vaccination strategies (pre-farrowing vs. post-farrowing) on antibody levels in sows and their piglets, using two different tests to assess changes in immunity.
- Results indicated that pre-farrowing vaccination led to higher antibody levels in both sows and their offspring compared to post-farrowing vaccination, with beneficial effects on piglet immunity lasting into the post-weaning period.
Exploring the wound healing potential of Lobostemon fruticosus using in vitro and in vivo bioassays.
J Ethnopharmacol
January 2025
Department of Pharmaceutical Sciences, Tshwane University of Technology, Private Bag X680, Pretoria, 0001, South Africa; SAMRC Herbal Drugs Research Unit, Faculty of Science, Tshwane University of Technology, Private Bag X680, Pretoria, 0001, South Africa. Electronic address:
Article Synopsis
- Lobostemon fruticosus is a traditional medicinal shrub from South Africa, known for treating skin issues like wounds, burns, and eczema, with reported anti-inflammatory and antibacterial properties.
- The study aimed to scientifically validate the wound healing abilities of L. fruticosus through experiments on both human cell lines and zebrafish larvae.
- The extract contained several compounds, including potential toxins at low levels, and showed promising results in enhancing cell migration in the scratch assay, indicating its potential for wound healing.
The impact of obesity on chronic oedema/lymphoedema of the leg - an international multicenter cross-sectional study (LIMPRINT).
Int J Obes (Lond)
September 2024
Department of Dermato-Venereology & Wound Healing Centre, Bispebjerg Hospital, Copenhagen University Hospital, Copenhagen, Denmark.
Article Synopsis
- Obesity and chronic oedema/lymphoedema are related conditions, with this study exploring how increased weight affects chronic oedema in patients with leg swelling.
- The study surveyed 7,397 patients across 38 centers in nine countries, revealing that heavier patients experienced more severe stages of chronic oedema compared to those with normal weight.
- Ten independent factors were linked to increased weight, including diabetes and decreased mobility, suggesting that obesity worsens chronic oedema control and decreases overall quality of life.
Factors associated with cellulitis in lymphoedema of the arm - an international cross-sectional study (LIMPRINT).
BMC Infect Dis
January 2024
Department of Dermato-Venereology & Wound Healing Centre, Bispebjerg Hospital, Bispebjerg Bakke 23, Copenhagen, 2400, Denmark.
Background: Lymphoedema is a globally neglected health care problem and a common complication following breast cancer treatment. Lymphoedema is a well-known predisposing factor for cellulitis, but few have investigated the risk factors for cellulitis in this patient cohort on an international level. The aim of this study was to identify the frequency of cellulitis in patients with lymphoedema of the arm, including potential risk factors for cellulitis.
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