A cDNA for the human "peripheral-type" benzodiazepine receptor (PBR) was isolated from a liver cDNA library. The 851-nucleotide probe hybridized with a approximately 1 kb mRNA in Northern blots of RNA extracted from various human tissues and cell lines. The human PBR probe was hybridized to DNA from a somatic cell hybrid mapping panel to determine that the gene maps to chromosome 22. With a regional mapping panel for chromosome 22, we localized the gene within band 22q13.31. The ligand-binding properties of the receptor expressed from the cDNA were examined in transient expression experiments and compared to the endogenous human PBR. The PBR ligand [3H]PK 11195 had high affinity for the expressed receptor in COS-1 cells, but the affinities of a pair of isoquinoline propanamide enantiomers differed remarkably in expressed and endogenous human PBR. These findings reveal that the host cell and/or post-translational modification may have an important influence on PBR function.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1089/dna.1992.11.471 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Total coliforms, E. coli, Enterococcus spp., and Staphylococcus spp. removal in vertical tubular photobioreactor with and without support medium.
Environ Pollut
December 2024
São Paulo State University (UNESP), School of Engineering Bauru, Department of Civil and Environmental Engineering, Bauru, SP, Brazil. Electronic address:
Unsafe water has severe implications for human health. Among sanitary wastewater treatment technologies, those that treat effluent in the most natural way possible (avoiding chemicals) need to be employed to minimize environmental damage upon release. Microalgae-based systems are one of the more economical and sustainable methods.
View Article and Find Full Text PDFComprehension of the adapted Urticaria Activity Score measure and patient guidance document: qualitative interviews with adults and adolescents with chronic spontaneous urticaria.
J Patient Rep Outcomes
December 2024
Institute of Allergology, Charité - Universitätsmedizin Berlin, Corporate Member of Freie Universität Berlin and Humboldt-Universität zu Berlin, Paul-Ehrlich-Haus, Hindenburgdamm 30, 12203, Berlin, Germany.
Objective: A key component of determining that a patient-reported outcome (PRO) measure is fit-for-purpose is to ensure that respondents understand its instructions and items. Any modification to a measure should be evaluated for relevance and understandability. The objective of the study was to assess comprehension of the adapted Urticaria Activity Score (UAS) questionnaire among adolescents aged populations with chronic spontaneous urticaria (CSU) and the modification to UAS question 2 to include patient-friendly terminology "wheals (hives).
View Article and Find Full Text PDFNon-stochastic reassembly of a metabolically cohesive gut consortium shaped by N-acetyl-lactosamine-enriched fibers.
Gut Microbes
December 2025
Department of Plant and Microbial Biology, North Carolina State University, Raleigh, NC, USA.
Diet is one of the main factors shaping the human microbiome, yet our understanding of how specific dietary components influence microbial consortia assembly and subsequent stability in response to press disturbances - such as increasing resource availability (feeding rate) - is still incomplete. This study explores the reproducible re-assembly, metabolic interplay, and compositional stability within microbial consortia derived from pooled stool samples of three healthy infants. Using a single-step packed-bed reactor (PBR) system, we assessed the reassembly and metabolic output of consortia exposed to lactose, glucose, galacto-oligosaccharides (GOS), and humanized GOS (hGOS).
View Article and Find Full Text PDFEffects of fishing restrictions on the recovery of the endangered Saimaa ringed seal (Pusa hispida saimensis) population.
PLoS One
December 2024
Department of Environmental and Biological Sciences, University of Eastern Finland, Joensuu, Finland.
Over the past three decades, incidental bycatch has been the single most frequent verified cause of death of the endangered Saimaa ringed seal (Pusa hispida saimensis). Spatial and temporal fishing closures have been enforced to mitigate bycatch, which is mainly caused by the gillnets of recreational fishers. In this study, we employed an array of statistical machine learning methods to recognize patterns of death and to evaluate the impacts of annual fishing closures (15th April-30th June) on the recovery of the Saimaa ringed seal population during 1991-2021.
View Article and Find Full Text PDFDeformable Mapping of Rectal Cancer Whole-Mount Histology with Restaging MRI at Voxel Scale: A Feasibility Study.
Radiol Imaging Cancer
November 2024
From the Departments of Radiology (J.M., J.N., T.H.K., L.R., M.J.G., N.H.), Surgery (J.S.H., J.C., M.G., J.G.A.), and Pathology (C.F., N.U., J.S.), Memorial Sloan-Kettering Cancer Center, 1275 York Ave, New York, NY 10065; Department of Radiology, University of São Paulo, São Paulo, Brazil (J.M., A.N.A., N.H.); Department of Medicine, Division of Precision Medicine, NYU Grossman School of Medicine, New York, NY (R.S.V.); Department of Biomedical Engineering, Vanderbilt University, Nashville, Tenn (J.S.H.); Research and Education Institute, Hospital Sirio-Libanes, São Paulo, Brazil (A.N.A.); and Department of Radiology, Mayo Clinic, Rochester, Minn (J.M., N.H.).
Purpose To develop a radiology-pathology coregistration method for 1:1 automated spatial mapping between preoperative rectal MRI and ex vivo rectal whole-mount histology (WMH). Materials and Methods This retrospective study included consecutive patients with rectal adenocarcinoma who underwent total neoadjuvant therapy followed by total mesorectal excision with preoperative rectal MRI and WMH from January 2019 to January 2022. A gastrointestinal pathologist and a radiologist established three corresponding levels for each patient at rectal MRI and WMH, subsequently delineating external and internal rectal wall contours and the tumor bed at each level and defining eight point-based landmarks.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!