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Characterisation of a second, apparently inactive, copy of the bovine beta-lactoglobulin gene.

Eur J Biochem

November 1995

School of Biochemistry and Molecular Genetics, University of New South Wales, Sydney, Australia.

A bovine cosmid clone was isolated which contains the previously characterised beta-lactoglobulin gene and, in addition, a related sequence which appears to be a beta-lactoglobulin pseudogene. The total length of the pseudogene, as determined by DNA sequencing, is 4.8 kb, similar to that of beta-lactoglobulin.

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The occurrence of the dominant 'whey' protein in samples of milk from 1180 sows is examined. It exhibits genetic polymorphism with some unusual features. Although immunologically different from bovine beta-lactoglobulin, it is shown by chemical studies of the isolated protein to be a beta-lactoglobulin.

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