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Picrotoxin-Induced Epileptogenic Hippocampal Organotypic Slice Cultures (hOTCs).
Methods Mol Biol
March 2025
The Neurogenesis, Neuroinflammation and Network Dynamics Lab (3ND), Achucarro Basque Center for Neuroscience, Leioa, Spain.
Cultured organotypic hippocampal slices (hOTCs) have become increasingly popular as a model for studying brain function. This model offers significant advantages over traditional in vitro methods, as they allow the examination of mid to long-term manipulations while preserving the structure of the dentate gyrus (DG) in the hippocampus. In this chapter, we focus on a protocol based on hOTCs of mouse entorhinal cortex and hippocampus, which by integrating techniques such as retroviral injections, immunohistochemistry, and microscopy imaging, physiological or pathological processes can be easily investigated.
View Article and Find Full Text PDFExperimental Neurogenesis in the Embryos of the Gecko Paroedura picta.
Methods Mol Biol
March 2025
Achucarro Basque Center for Neuroscience, Scientific Park of the University of the Basque Country (UPV/EHU), Leioa, Spain.
This research introduces innovative tools for studying embryonic neurogenesis in the gecko Paroedura picta. Traditional research methods have been adapted for the gecko's unique biology, including variations of birthdating techniques and the implementation of EdU for tracking neuron generation. We also employ hybridization chain reaction (HCR) to detect specific mRNAs without the need for species-specific antibodies, providing a powerful and flexible tool for studying gene expression patterns in the gecko embryonic brain.
View Article and Find Full Text PDFBmal1 knockout aggravates Porphyromonas gingivalis-induced periodontitis by activating the NF-κB pathway.
J Appl Oral Sci
February 2025
Sichuan University, West China Hospital of Stomatology, National Clinical Research Center for Oral Diseases, State Key Laboratory of Oral Diseases, Department of General Clinic, Chengdu, China.
Background: Circadian rhythm disorders and NF-κB are closely linked and can exacerbate periodontitis. However, the mechanisms via which circadian rhythm-related genes influence periodontitis are not yet fully understood.
Objective: We investigated the effect of brain and muscle Arnt-like protein-1 (BMAL1) on the NF-κB pathway and downstream inflammatory factors on periodontitis.
Direct Cryosectioning of Drosophila Heads for Enhanced Brain Fluorescence Staining and Immunostaining.
J Vis Exp
February 2025
Department of Pathology, Division of Molecular and Cellular Pathology, Heersink School of Medicine, The University of Alabama at Birmingham; UAB Nathan Shock Center;
Immunostaining Drosophila melanogaster brains is essential for exploring the mechanisms behind complex behaviors, neural circuits, and protein expression patterns. Traditional methods often involve challenges such as performing complex dissection, maintaining tissue integrity, and visualizing specific expression patterns during high-resolution imaging. We present an optimized protocol that combines cryosectioning with fluorescence staining and immunostaining.
View Article and Find Full Text PDFDifferential Expression of MRPS18 Family Genes in Brain Tumor Samples.
Exp Oncol
February 2025
R.E. Kavetsky Institute of Experimental Pathology, Oncology and Radiobiology, NASU, Kyiv, Ukraine.
Background: Brain tumors account for 2%-3% of all malignant neoplasms and 85%-90% of all primary tumors of the central nervous system with the 5-year survival rate of 35%. Additional biomarkers could help refine the molecular profile of brain tumors and prognosis of the disease.
Aim: To study differential expression patterns of the MRPS18 family genes in tumor tissue and the peripheral blood of patients with brain tumors of various types.
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