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Characterization of the Sodium Multi-Vitamin Transporter in the Mosquito and Its Capacity to Mobilize Pantothenate and Biotin.
Biomolecules
January 2025
Department of Entomology, University of Arizona, Tucson, AZ 85721, USA.
Pantothenate (Pan), or vitamin B5, is essential for the synthesis of co-enzyme A (CoA), acetyl-CoA, and numerous downstream physiological processes. We previously demonstrated that Pan is not only essential for mosquito survival, but also for the development of malaria parasites within the mosquito, suggesting that targeting Pan and CoA biosynthesis may be a novel approach for malaria control. However, little is known about how Pan is acquired and mobilized within the mosquito.
View Article and Find Full Text PDFRapid Determination of Water-Soluble Vitamins in Human Serum by Ultrahigh-Performance Liquid Chromatography-Tandem Mass Spectrometry.
ACS Omega
January 2025
Clinical Nuclear Medicine Center, Imaging Clinical Medical Center, Institute of Nuclear Medicine, Institute of Clinical Mass Spectrometry Applied Research Center, Department of Nuclear Medicine, Shanghai Tenth People's Hospital, School of Medicine, Tongji University, Shanghai 200072, China.
Water-soluble vitamins play essential roles in normal body functions and metabolic activities. However, few methods have simultaneously measured all nine water-soluble vitamins in biological matrices. In this study, we developed a sensitive and accurate method for the simultaneous measurement of thiamine (B1), riboflavin (B2), nicotinamide (B3), pantothenic acid (B5), 4-pyridoxic acid (B6), biotin (B7), 5-methyltetrahydrofolic acid (B9), ascorbic acid (VC), and methylmalonic acid (MMA) in human serum.
View Article and Find Full Text PDFUntargeted LC-HRMS analyses reveal metabolomic specificities between wine yeast strains selected for their malic acid production.
Food Chem
December 2024
BIOLAFFORT, 11 rue Aristide Berges, 33270 Floirac, France; UMR OENO, Université de Bordeaux, INRAE, INP, BSA, ISVV, 210 Chemin de Leysotte, 33882 Villenave d'Ornon, France. Electronic address:
The alcoholic fermentation of wine is mostly achieved by the species Saccharomyces cerevisiae that display a large variability for their ability to consume or produce malic acid. To better characterize the metabolism of such group of strains we explored their non-volatile metabolome using an untargeted LC-HRMS approach. The chemical classes and the putative structures of several hundred compounds where annotated using MS2 spectra using the SIRIUS software.
View Article and Find Full Text PDFAntibacterial mechanism of SHY96 cell-free supernatant against revealed by metabolomics and potential application on chicken breast meat preservation.
Food Chem X
January 2025
College of Food Science, Southwest University, Chongqing 400715, China.
The cell-free supernatant of (LCFS) is considered a potential natural antimicrobial agent due to its outstanding antimicrobial activity. This study demonstrated that the cell-free supernatant of SHY96 (LCFS96) effectively inhibits the growth and biofilm formation of CMCC(B)54002 (_02) by reducing cell metabolic activity and damaging cell structure. Metabolomic analysis revealed that LCFS96 significantly altered 450 intracellular metabolites, affecting key metabolic pathways including linoleic acid metabolism, pyrimidine metabolism, purine metabolism, pantothenic acid and CoA biosynthesis, and the TCA cycle.
View Article and Find Full Text PDFExploration of key genes and pathways in response to submergence stress in red clover (Trifolium pratense L.) by WGCNA.
BMC Plant Biol
January 2025
College of Animal Science and Technology, Southwest University, Chongqing, China.
Background: Submergence stress is a prevalent abiotic stress affecting plant growth and development and can restrict plant cultivation in areas prone to flooding. Research on plant submergence stress tolerance has been essential in managing plant production under excessive rainfall. Red clover (Trifolium pratense L.
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