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Application of an anticoagulant to prevent clotting is a prerequisite for the retention of morphological integrity of platelets in blood samples. Among the anticoagulants used for the collection of blood from veterinary animals, are heparin, the oxalate salts (sodium and potassium) and di-sodium and tri-sodium citrates. In a study on the effects of anticoagulants on platelet morphology, it was noted that oxalates caused severe morphological distortion in platelets.
View Article and Find Full Text PDFBacillus cereus was used as the assay organism for the quantification of chlortetracycline (CTC) in avian plasma. Antibiotic medium #8 gave significantly larger zones of inhibition than nutrient agar 1.5% when used as the assay medium (P less than or equal to 0.
View Article and Find Full Text PDFThe reaction-rate (Dade) and clot-density (Sherwood) methods for determining plasma fibrinogen were compared. Both require small sample volumes, are economical, and may be performed using commercially standardized instruments and reagents. Equivalent times were required for sample preparation.
View Article and Find Full Text PDF1. The use of Evans Blue dye to facilitate endpoint determination the elimination of 4 degrees, assay conditions are technical improvements in the euglobulin clot lysis test. 2.
View Article and Find Full Text PDFOptimal amounts of demineralized bone matrix possess the ability to coagulate platelet-free heparinized, citrated, and oxalated blood plasmas of guinea pigs. Clotting constituents become denatured in contact with the insoluble coagulant proteins. Quantities in excess of optimal modify plasma so that it does not gel when thrombin is added.
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