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Description of the Serological Response After Treatment of Chronic Imported Schistosomiasis.
Trop Med Infect Dis
January 2025
National Reference Centre for Imported Tropical Diseases, Infectious Diseases Department, Hospital Universitario Ramón y Cajal, IRYCIS, 28034 Madrid, Spain.
Background: Chronic schistosomiasis can lead to significant morbidity. Serology is highly sensitive; however, its role in assessing treatment response is controversial. This study aimed to analyze serological values following treatment of chronic imported schistosomiasis.
View Article and Find Full Text PDFA new way of the Coombs test using flow cytometry-based assay to assess erythrocytes-bound IgG antibodies in the human and rabbit model.
Int J Immunopathol Pharmacol
January 2025
College of Medical Laboratory, Dalian Medical University, Dalian, Liaoning, China.
The Coombs test is important in hematology for detecting erythrocyte-bound IgG antibodies or in serm through agglutination methods, but its sensitivity and specificity are limited. Flow cytometry provides a more precise and sensitive alternative for quantitatively assessing RBC-bound IgG antibodies. This assessment is crucial for evaluating the risk of hemolytic reactions and ensuring safe transfusions.
View Article and Find Full Text PDFTiming of Assessment of Humoral and Cell-Mediated Immunity after Influenza Vaccination.
Vaccines (Basel)
May 2024
Department of Microbiology, Hyogo Medical University, Nishinomiya 663-8501, Hyogo, Japan.
Assessment of the immune response to influenza vaccines should include an assessment of both humoral and cell-mediated immunity. However, there is a lack of consensus regarding the timing of immunological assessment of humoral and cell-mediated immunity after vaccination. Therefore, we investigated the timing of immunological assessments after vaccination using markers of humoral and cell-mediated immunity.
View Article and Find Full Text PDFDoes strengths of a positive direct antiglobulin test predicts the need for phototherapy and duration of phototherapy? - a single center, retrospective study.
J Matern Fetal Neonatal Med
December 2023
Transfusion, South Tees Pathology, James Cook University Hospital, Middlesbrough, UK.
Background: Use of Direct Antiglobulin test (DAT) in management of neonatal hyperbilirubinemia is conflicting.
Objective: whether strength of positive DAT predicts the need for phototherapy, duration of phototherapy and need for major interventions.
Methods: We retrospectively collected data on all DAT positive neonates with birth gestational age ≥32 weeks over six years (2014-2019).
Better resolving of anti-CD38 antibody interference with blood compatibility testing by using manual polybrene method compared with dithiothreitol-pretreatment indirect antiglobulin test.
J Clin Lab Anal
April 2023
Division of Hematology and Oncology, Department of Internal Medicine, China Medical University Hospital, Taichung, Taiwan.
Background: It is advised to pretreat the reagent erythrocytes with Dithiothreitol (DTT) to denature the surface CD38 to prevent anti-CD38 monoclonal antibodies (MoAb) from interfering with the blood compatibility test. Anti-CD38 has little impact on the Polybrene test, but it is still unknown how sensitive it is to detect irregular antibodies and how effective it is when compared to the standard DTT-based method.
Methods: Twenty-one patients receiving daratumumab (N = 13) and isatuximab (N = 8) had their serum collected.
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