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Cryogenic, but not hypothermic, preservation disrupts the extracellular matrix of cell sheets.
Bioact Mater
April 2025
3B's Research Group, I3Bs - Research Institute on Biomaterials, Biodegradables and Biomimetics, University of Minho, Headquarters of the European Institute of Excellence on Tissue Engineering and Regenerative Medicine, AvePark, Parque de Ciência e Tecnologia, Rua Ave 1, Edifício 1 (Sede), 4805-694 Barco, Guimarães, Portugal.
Cell sheet (CS)-based approaches hold significant potential for tissue regeneration, relying on the extracellular matrix (ECM) for success. Like in native tissues, the ECM provides structural and biochemical support for cellular homeostasis and function. Effective preservation strategies that maintain ECM integrity are critical to enhance the therapeutic potential of CS-based approaches.
View Article and Find Full Text PDFReverse transcriptase inhibitors diminish systemic proinflammatory responses to bacterial pathogens.
mBio
January 2025
Department of Microbiology, Harvard Medical School, Boston, Massachusetts, USA.
Bacterial infections can induce exuberant immune responses that can damage host tissues. Previously, we demonstrated that systemic infection in mice causes tissue damage in the liver. This liver necrosis is associated with the expression of endogenous retroviruses, chromosomally integrated retroviruses that encode a reverse transcriptase.
View Article and Find Full Text PDFDuration of Hypothermia is Associated with Postoperative Complications in Patients Undergoing Gynecological Surgery: A Prospective Cohort Study.
Chin Med Sci J
December 2024
Department of Anesthesiology.
Objectives: To investigate the relationship between hypothermia duration and postoperative complications in patients undergoing gynecological surgery.
Methods: Patients who underwent elective gynecological surgery at our hospital were consecutively enrolled between October 2020 and January 2022. Core temperature was continuously monitored intraoperatively, and early postoperative complications were collected.
Comparison of the lethal triad and the lethal diamond in severe trauma patients: a multicenter cohort.
World J Emerg Surg
January 2025
Federation of Anesthesiology, Intensive Care Unit, Burns and Operating Theater, Percy Military Training Hospital, 2 Rue Lieutenant Raoul Batany, 92140, Clamart, France.
Background: To reduce the number of deaths caused by exsanguination, the initial management of severe trauma aims to prevent, if not limit, the lethal triad, which consists of acidosis, coagulopathy, and hypothermia. Recently, several studies have suggested adding hypocalcemia to the lethal triad to form the lethal diamond, but the evidence supporting this change is limited. Therefore, the aim of this study was to compare the lethal triad and lethal diamond for their respective associations with 24-h mortality in severe trauma patients receiving transfusion.
View Article and Find Full Text PDFEffect of Superficial Large Vessel Insulation Combined with Self-heating Warm Paste on Intraoperative Hypothermia in Patients Undergoing Urologic Surgery.
Arch Esp Urol
December 2024
Surgical Complex, Taizhou Hospital of Zhejiang Province Affiliated to Wenzhou Medical University, 317000 Taizhou, Zhejiang, China.
Objective: This study aimed to explore the application effect of superficial large vessel insulation combined with self-heating warm paste on intraoperative hypothermia in patients undergoing urologic surgery.
Methods: This study included 313 patients who underwent urologic surgery in Taizhou Hospital of Zhejiang Province Affiliated to Wenzhou Medical University from May 2023 to March 2024. After 13 patients with incomplete clinical data, abnormal basal metabolism and preoperative skin injury were excluded, 300 patients were finally included.
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