Objective: To develop a simple and efficient method for extracting Gelsemium alkaloids.
Methods: Uniform design and orthogonal design were respectively utilized to optimize the 3 factors in the extraction process, namely the type of solvent used, the solid-to-liquid ratio in the extraction system and the reflux time.
Results: Both of the design methods adopted trichloromethane as the solvent, with the solid-to-liquid ratio of 1:7.5 and reflux time of 3 h with 3 repetitions for the highest yield of alkaloids.
Conclusion: Similar results are obtained from the two design methods for extracting Gelsemium alkaloids.
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