1. The existence of both cAMP-responsive element binding factor and a nuclear factor 1-like (NF-1-like) protein in nuclear extracts from liver of cAMP-treated rat has been revealed. 2. Binding of these proteins to a DNA fragment containing both elements was cooperative, and 50% binding was achieved with considerably less protein than with a fragment bearing either element alone. 3. Cleavage of the fragment between the two elements abolished the apparent cooperative interaction. 4. Southwestern blot analysis showed that the NF-1-like protein has a molecular weight in the 28-30-kDa range. 5. The NF-1-like binding activity was very stable.
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