AI Article Synopsis
- The study investigated the impact of human hepatocyte growth factor (hHGF) on the growth of human liver cancer cells.
- Out of five examined cell lines, only HuH-6 Clone 5 cells showed increased proliferation in response to hHGF.
- Both natural and synthetic forms of hHGF boosted cell growth and DNA synthesis, but this effect was significantly blocked by an anti-hHGF monoclonal antibody.
Article Abstract
The effects of human hepatocyte growth factor (hHGF), a potent mitogen for rat and human hepatocytes in primary culture, on proliferation of human hepatoma and hepatoblastoma cells were examined. Out of five cell lines; HLE, HuH-6 clone 5, HuH-7, PLC/PRF/5, and Hep G2, only HuH-6 Clone 5 cells were stimulated by recombinant hHGF. Both native and recombinant hHGFs caused dose-dependent increases in cell number and DNA synthesis of cells. This stimulation was strongly inhibited by anti-hHGF monoclonal antibody.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1016/s0309-1651(06)80108-6 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Serabelisib regulates GSDMD-mediated pyroptosis, apoptosis and migration of hepatoma cells via the PI3K/Akt/E-cadherin signaling pathway.
Adv Clin Exp Med
February 2024
Second Department of General Surgery, Hunan Children's Hospital, Changsha, China.
Background: Liver cancer is a malignant tumor commonly seen in infants and young children. Serabelisib is a novel and effective phosphoinositide-3-kinase (PI3Kα) inhibitor, currently in trials for solid malignancy treatment, such as bladder cancer. However, it is unclear whether serabelisib affects liver cancer.
View Article and Find Full Text PDFArctigenin induces apoptosis in human hepatoblastoma cells.
Pediatr Surg Int
June 2019
Pediatric Surgery Department, Fujita Health University, Toyoake, Japan.
Purpose: Arctigenin has been shown to have anti-tumor effects in various types of cancers. This study was conducted to verify these effects in the human-derived hepatoblastoma cell line, HUH-6 clone 5 (hereinafter, HUH-6).
Methods: Arctigenin was added to cultured HUH-6 cells, and cellular activity was evaluated by MTS assay.
Detection of CD133 (prominin-1) in a human hepatoblastoma cell line (HuH-6 clone 5).
Microsc Res Tech
August 2013
Division of Morphological Science, Biomedical Research Center, Saitama Medical University, Iruma-gun, Saitama, Japan.
We examined CD133 distribution in a human hepatoblastoma cell line (HuH-6 clone 5). We directly observed the cultured cells on a pressure-resistant thin film (silicon nitride thin film) in a buffer solution by using the newly developed atmospheric scanning electron microscope (ASEM), which features an open sample dish with a silicon nitride thin film window at its base, through which the scanning electron microscope beam scans samples in solution, from below. The ASEM enabled observation of the ventral cell surface, which could not be observed using standard SEM.
View Article and Find Full Text PDFIsolation and identification of cancer stem cells from a side population of a human hepatoblastoma cell line, HuH-6 clone-5.
Pediatr Surg Int
January 2011
Department of Pediatric Surgery, Saitama Medical University, Iruma-gun, Saitama, 350-0495, Japan,
Purpose: It has been thought that the persistence of even a small number of tumor cells in the body may increase each tumor cell in a similar manner and may allow the disease to proceed. However, only a few percent of such tumor cells exist in cancerous tissue. They are called "cancer stem cells (CSCs)".
View Article and Find Full Text PDFA REIC gene shows down-regulation in human immortalized cells and human tumor-derived cell lines.
Biochem Biophys Res Commun
February 2000
Department of Cell Biology, Institute of Molecular and Cellular Biology, Okayama University Medical School, Shikata-cho 2-5-1, Okayama, 700-8558, Japan.
Normal human cells stop proliferation after a certain number of cell divisions. This phenomenon is called cellular aging. The fact that the senescence phenotype is dominant and the immortal one is recessive indicates that immortalization of human cells may be caused by loss of functions of certain genes in normal cells.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!