Download full-text PDF |
Source |
|---|---|
| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC312043 | PMC |
| http://dx.doi.org/10.1093/nar/20.4.916 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
MARTRE family proteins negatively regulate CCR4-NOT activity to protect poly(A) tail length and promote translation of maternal mRNA.
Nat Commun
January 2025
Key Laboratory of Epigenetic Regulation and Intervention, Institute of Biophysics, Chinese Academy of Sciences, Beijing, 100101, China.
Article Synopsis
- The study focuses on the role of a newly discovered protein family called MARTRE in regulating the poly(A) tail length of maternal mRNA during early embryo development in mice.
- MARTRE proteins inhibit the deadenylase CCR4-NOT, helping to maintain longer poly(A) tails and enhance mRNA translation efficiency.
- Deleting the Martre genes leads to shortened poly(A) tails, reduced mRNA translation, and delays in early embryonic development, emphasizing the importance of MARTRE in the translation of maternal mRNA.
RNA 3'end tailing safeguards cells against products of pervasive transcription termination.
Nat Commun
December 2024
Department of Molecular Biology and Genetics, Aarhus University, Aarhus, Denmark.
Article Synopsis
- - Premature transcription termination leads to the accumulation of unadenylated RNA, which the NEXT complex typically degrades, but alternative degradation pathways are not well understood.
- - Upon inactivation of NEXT, there is an increase in 3' end uridylated and adenylated RNAs, with short U-tailed RNAs modified by TUT4/7 and longer RNAs adenylated by enzymes like TENT2.
- - These RNAs are degraded through different mechanisms, including the PAXT pathway in the nucleus or the cytoplasmic exosome, where failure to degrade them can result in reduced translation and cell death.
Detection of Nuclear RNA Decay Intermediates Using a Modified Oxford Nanopore RNA Sequencing Strategy.
Methods Mol Biol
November 2024
Department of Chemistry and Biochemistry and the Molecular Biology Institute, University of California Los Angeles, Los Angeles, CA, USA.
The nuclear RNA exosome complex is crucial for noncoding RNA processing and RNA quality control in the nucleus. Identifying substrates and intermediates of RNA decay pathways, such as those mediated by the exosome complex using Oxford Nanopore sequencing can be difficult in part because a simple method to detect them has been lacking and also because some of these RNAs lack abundant poly(A) tails which are required for Oxford Nanopore-based sequencing. Here we describe an Oxford nanopore-based approach which can be used to identify long reads corresponding to precursors and products of nuclear exosome processing.
View Article and Find Full Text PDFDynamic Evolution of Poly-A Tail Lengths Visualized by RNAse H Assay and Northern Blot Using Nonradioactive Probes in Yeast.
Methods Mol Biol
November 2024
Department of Microbiology and Molecular Medicine, Faculty of Medicine, University of Geneva, Institute of Genetics and Genomics of Geneva, Geneva, Switzerland.
Poly-A tail length dynamics have been extensively studied from yeast to human, mostly using reporter transcripts. Recent studies have been carried out genome-wide to determine the status of poly-A tails at steady state. However, poly-A tail measurement at equilibrium gives an overall length that reflects a mixture of the different poly-A tail sizes for a single transcript.
View Article and Find Full Text PDFEstrogen receptor alpha (ERα) regulates PARN-mediated nuclear deadenylation and gene expression in breast cancer cells.
RNA Biol
January 2024
Chemistry Department, Hunter College, The City University of New York, New York, NY, USA.
Article Synopsis
- The study investigates the dynamic role of estrogen receptor alpha (ERα) in breast cancer, particularly in the process of mRNA 3' end processing, which affects gene expression after transcription.
- It reveals that ERα activates the enzyme poly(A) specific ribonuclease (PARN) in collaboration with the tumor suppressor p53, enhancing its role in mRNA processing.
- The research identifies shared mRNA targets between ERα and PARN related to cell invasion and metastasis, suggesting a novel way ERα influences gene expression beyond traditional transcriptional mechanisms, contributing to breast cancer progression.
Want AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!