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AGEing of collagen: The effects of glycation on collagen's stability, mechanics and assembly.
Matrix Biol
February 2025
Department of Physics, Simon Fraser University, Burnaby, BC, V5A 1S6, Canada. Electronic address:
Advanced Glycation End Products (AGEs) are the end result of the irreversible, non-enzymatic glycation of proteins by reducing sugars. These chemical modifications accumulate with age and have been associated with various age-related and diabetic complications. AGEs predominantly accumulate on proteins with slow turnover rates, of which collagen is a prime example.
View Article and Find Full Text PDFDiscovery and mechanistic exploration of promiscuous xylosyltransferase based on protein engineering.
Int J Biol Macromol
January 2025
National Resource Center for Chinese Meteria Medica, China Academy of Chinese Medical Sciences, Beijing 100700, China; State Key Laboratory for Quality Ensurance and Sustainable Use of Dao-di Herbs, Beijing 100700, China.
Glycosylation is an effective means to alter the structure and properties of plant compounds, influencing the pharmacological activity of natural products (NPs) to obtain highly active NPs. In nature, glucosides are the most widely distributed, while other glycosides such as xylosides are less common and present in lower quantities. This is due to the scarcity of xylosyltransferases with substrate promiscuity in nature, and the modification of their catalytic function is also quite challenging.
View Article and Find Full Text PDFFragment-specific Quantification of 5hmC by qPCR via a Combination of Enzymatic Digestion and Deamination: Extreme Specificity, High Sensitivity, and Clinical Applicability.
Anal Chem
January 2025
School of Biomedical Engineering, Shanghai Jiao Tong University, Shanghai 200240, China.
Accurate identification and quantification of 5-hydroxymethylcytosine (5hmC) can help elucidate its function in gene expression and disease pathogenesis. Current 5hmC analysis methods still present challenges, especially for clinical applications, such as having a risk of false-positive results and a lack of sufficient sensitivity. Herein, a 5hmC quantification method for fragment-specific DNA sequences with extreme specificity, high sensitivity, and clinical applicability was established using a quantitative real-time PCR (qPCR)-based workflow through the combination of enzymatic digestion and biological deamination strategy (EDD-5hmC assay).
View Article and Find Full Text PDFProtection of Enzymes Against Heat Inactivation by Enzyme-Polymer Conjugates.
Macromol Rapid Commun
January 2025
State Key Lab of Polymer Materials Engineering, School of Chemical Engineering, Sichuan University, Chengdu, 610065, China.
Along with the quick advancements in enzyme technology, inactivation has emerged as the key barrier for enzymes to be fully utilized as biocatalysts. Here, a novel strategy is presented for the preservation of the enzymatic activity even after heat treatment by grafting enzymes onto the thermal responsive block copolymer via an activated ester-amine reaction. A new water-soluble activated ester monomer, acrylic polyethylene glycol (PEG) functionalized 3-fluoro-4-hydroxybenzoate is synthesized.
View Article and Find Full Text PDFRelationship Between Physical Characteristics of Cereal Polysaccharides and Soft Tribology-The Importance of Grain Source and Malting Modification.
Food Sci Nutr
January 2025
Technical University of Munich, TUM School of Life Sciences, Chair of Brewing and Beverage Technology, Group Raw Material Based Brewing and Beverage Technology Freising Germany.
Starch and non-starch polysaccharides ((N)SPs) are relevant in cereal-based beverages. Although their molar mass and conformation are important to the sensory characteristics of beer and non-alcoholic beer, their triggering mechanism in the mouth is not fully understood. Soft tribology has emerged as a tool to mimic oral processing (drinking).
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