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Copper-dependent halogenase catalyses unactivated C-H bond functionalization.
Nature
January 2025
Department of Chemical and Biomolecular Engineering, University of California, Los Angeles, Los Angeles, CA, USA.
Carbon-hydrogen (C-H) bonds are the foundation of essentially every organic molecule, making them an ideal place to do chemical synthesis. The key challenge is achieving selectivity for one particular C(sp)-H bond. In recent years, metalloenzymes have been found to perform C(sp)-H bond functionalization.
View Article and Find Full Text PDFConstruction of a Multiple Cyclic Ligation-Promoted Exponential Recombinase Polymerase Amplification Platform for Sensitive and Simultaneous Monitoring of Cancer Biomarkers Fpg and FEN1.
Anal Chem
January 2025
School of Chemistry and Chemical Engineering, State Key Laboratory of Digital Medical Engineering, Southeast University, Nanjing 211189, China.
Formamidopyrimidine DNA glycosylase (Fpg) and flap endonuclease 1 (FEN1) are essential to sustaining genomic stability and integrity, while the abnormal activities of Fpg and FEN1 may lead to various diseases and cancers. The development of simple methods for simultaneously monitoring Fpg and FEN1 is highly desirable. Herein, we construct a multiple cyclic ligation-promoted exponential recombinase polymerase amplification (RPA) platform for sensitive and simultaneous monitoring of Fpg and FEN1 in cells and clinical tissues.
View Article and Find Full Text PDFReusable Self-Powered Electrochromic Sensor Patch Based on Enzymatic Biofuel Cells for On-Site Visualized Monitoring of Lactic Acid.
Anal Chem
January 2025
School of Chemistry and Chemical Engineering, Jiangsu University, Zhenjiang 212013, PR China.
Wearable sensors have broad application potential in motion assessment, health monitoring, and medical diagnosis. However, relying on a specialized instrument for power supply and signal reading makes sensors unsuitable for on-site detection. To solve this problem, a reusable self-powered electrochromic sensor patch based on enzymatic biofuel cells were constructed to realize the on-site visualized monitoring.
View Article and Find Full Text PDFMicroplastics in Cuban freshwaters: diversity, temporal changes, and effects on extracellular enzymatic activity.
Environ Pollut
January 2025
Universidad de Alcalá, Departamento de Química Analítica, Química Física e Ingeniería Química, Ctra. Madrid-Barcelona Km. 33.600, 28871, Alcalá de Henares, Madrid, Spain.
Plastics, as synthetic polymers, are emerging contaminants that can harm organisms and ecosystems. This study investigates the presence of microplastics in sediments of two rivers in western Cuba, assessing their temporal variability, diversity, and characterizing the types of microplastics in these ecosystems. Additionally, the study examines the relationship between microplastic concentrations, the extracellular enzymatic activity of benthic microbial communities, and nutrient levels in sediments.
View Article and Find Full Text PDFCofactor-directed co-immobilization of dual-enzyme on functionalized montmorillonite with enhanced catalytic performance.
Int J Biol Macromol
January 2025
School of Environment and Resource, Key Laboratory of Solid Waste Treatment and Resource Recycle of Ministry of Education, Southwest University of Science and Technology, Mianyang, Sichuan 621010, China.
Recently, multi-enzyme cascade catalysis has attracted increasing attention due to the advantages of integrating multiple enzymes, few side reactions and high catalytic efficiency. Herein, a novel dual-enzyme cascade system (GOx-FMt-HRP) was developed through cofactor-directed orientational co-immobilization of glucose oxidase (GOx) and horseradish peroxidase (HRP) onto functional montmorillonite (FMt). The presented method realizes the reconstitution of cofactors and apo-enzymes (enzymes without cofactors), which enables enzymes to be immobilized in specific orientations on the support, thereby effectively reducing changes in their conformation.
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