The XhoI-SalGI fragment of the plasmid pCI DNA was inserted into the SalGI site of the cyanobacterium Anacystis nidulans R2 integrative vector plasmid pIAH4. The fragment incorporates the endoglucanase gene of Clostridium thermocellum cloned earlier within the 6.7 kb DNA sequence. The recombinant plasmid DNA was transformed into Anacystis nidulans R2 cells. The cloned endoglucanase gene was shown to express in the cyanobacterium cells. The enzyme synthesized is accumulated within the cytoplasm of Anacystis nidulans cells and is not secreted into the periplasm.
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Biotechnol Biofuels Bioprod
March 2023
School of Basic Medical Sciences, Lanzhou University, Lanzhou, 730000, China.
Background: Artificial microbial consortia composed of heterotrophic and photoautotrophic organisms represent a unique strategy for converting light energy and carbon dioxide into high-value bioproducts. Currently, the types of desired bioproducts are still limited, and microbial fitness benefit rendered by paired partner generally needs to be intensified. Exploring novel artificial microbial consortia at a laboratory scale is an essential step towards addressing this unmet need.
View Article and Find Full Text PDFNucleic Acids Res
June 2022
Anhui Province Key Laboratory of Active Biological Macro-molecules, Wannan Medical College, Wuhu, Anhui 241002, China.
Synechococcus elongatus, formerly known as Anacystis nidulans, is a representative species of cyanobacteria. It is also a model organism for the study of photoreactivation, which can be fully photoreactivated even after receiving high UV doses. However, for a long time, only one photolyase was found in S.
View Article and Find Full Text PDFBiophys Physicobiol
September 2020
Center for Biosystems Dynamics Research, RIKEN, Suita, Osaka 565-0874, Japan.
Following the discovery of cryptochrome-DASH (CRYD) as a new type of blue-light receptor cryptochrome, theoretical and experimental findings on CRYD have been reported. Early studies identified CRYD as highly homologous to the DNA repair enzyme photolyases (PLs), suggesting the involvement of CRYD in DNA repair. However, an experimental study reported that CRYD does not exhibit DNA repair activity .
View Article and Find Full Text PDFN Z J Bot
December 2018
Division of Biological Sciences, University of California - San Diego, La Jolla, California USA, 9500 Gilman Drive #0116, La Jolla, CA 92093-0116, +1-858-246-0658,
This perspective provides a historical account of the isolation and nomenclature of the cyanobacterial strains currently known as . The story focuses on an isolate from the San Francisco Bay area of California (Pasteur Culture Collection PCC 7942) that has, for decades, been the genetic model for this species, and its close relative isolated from Waller Creek in Texas (PCC 6301, also known as the University of Texas at Austin Culture Collection of Algae UTEX 625). Until recently, these strains have been the only representatives of the species.
View Article and Find Full Text PDFToxins (Basel)
July 2019
Department of Genetics, Development and Molecular Biology, School of Biology, Aristotle University of Thessaloniki, GR-541 24 Thessaloniki, Greece.
Cyanobacteria are a diverse group of photosynthetic Gram-negative bacteria that produce an array of secondary compounds with selective bioactivity against a broad spectrum of organisms and cell lines. In this study, 29 strains isolated from freshwaters in Greece were classified using a polyphasic approach and assigned to Chroococcales, Synechococcales, and Nostocales, representing 11 genera and 17 taxa. There were good agreements between 16S ribosomal RNA (rRNA)--internal genetic spacer (IGS) characterization and morphological features, except for the - group which appears intermixed and needs further elucidation.
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