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Supplementation of DHA enhances the cryopreservation of yak semen via alleviating oxidative stress and inhibiting apoptosis.
Front Vet Sci
February 2025
Key Laboratory for Animal Science of National Ethnic Affairs Commission, Southwest Minzu University, Chengdu, China.
Introduction: Semen cryopreservation is a crucial method for preserving genetic resources and accelerating the breeding process in domestic animals. However, the frozen-thawed process often leads to physical and chemical damage in semen, resulting in oxidative stress that diminishes sperm vitality and fertilization potential. This study aimed to explore the effects of docosahexaenoic acid (DHA) on the quality of frozen-thawed yak semen.
View Article and Find Full Text PDFSize-Exclusion Chromatography of Macromolecules: A Brief Tutorial Overview on Fundamentals with Computational Tools for Data Analysis and Determination of Structural Information.
Polymers (Basel)
February 2025
Department of Physical Chemistry, University of Murcia, 30100 Murcia, Spain.
Size-exclusion chromatography (SEC) is presently a widely used and very informative technique for the characterization of macromolecules in solution. Beyond the first implementations of SEC-which required cumbersome column calibrations and were mainly intended for the determination of molecular weights-the modern SEC approach involving multiple detectors (md-SEC) is based on solution properties such as intrinsic viscosity and light scattering. Thus, md-SEC enables the direct and more efficient determination of molecular weights, as well as the determination of relationships between property and molecular weight, which can be quite useful in structural studies.
View Article and Find Full Text PDFWhole-genome sequencing-based species classification, multilocus sequence typing, and antibiotic resistance mechanisms of the clinical complex.
Front Microbiol
February 2025
Key Laboratory of Medical Genetics of Zhejiang Province, Key Laboratory of Laboratory Medicine, Ministry of Education, China, School of Laboratory Medicine and Life Sciences, Wenzhou Medical University, Wenzhou, China.
Background: Multidrug-resistant strains of the genus can produce various β-lactamases that confer resistance to a broad spectrum of β-lactams, which poses a significant public health threat due to their emergence and spread in clinical settings and natural environments. Therefore, a comprehensive investigation into the antibiotic resistance mechanisms of is scientifically significant.
Methods: Between 2018 and 2021, 78 clinical isolates were collected from human clinical specimens.
Development of an enzyme-linked immunosorbent assay (ELISA) for determining neutrophil elastase (NE) - a potential useful marker of multi-organ damage observed in COVID-19 and post-Covid-19 (PCS).
Front Mol Biosci
February 2025
Clinical Department of Diabetology, Hypertension and Internal Disease, Wroclaw Medical University, Wroclaw, Poland.
Background: The ongoing post-COVID-19 syndrome (PCS) epidemic, causing complications of diverse etiology, necessitates the search for new diagnostic markers and the development of widely accessible methods for their detection. This would enable the prognosis of PCS progression and faster implementation of targeted treatments. One potential marker is neutrophil elastase (NE), whose elevated levels in the blood during PCS may result from organ damage caused by increased secretion of severe inflammatory mediators or amyloidosis resulting from the interaction of NE with SARS-CoV-2.
View Article and Find Full Text PDFEffects of different concentrations of chlormequat chloride on bacterial community composition and diversity in peanut soil.
BMC Microbiol
March 2025
Institute of Agricultural Quality Standards and Testing Technology, Liaoning Academy of Agricultural Sciences, Shenyang, China.
The application of pesticides may have significant impacts on soil environment and communities. In order to understand the deep relationship between the application of chlormequat chloride (CC) and the bacterial community in peanut soil, high-resolution characterization was performed using peanut soil samples (12 points; 0-20 cm rhizosphere soil) from untreated and sprayed with different concentrations of CC. Experimental data showed that with the increase of concentration, operational taxonomic units (OTUs) richness showed a decreasing tendency.
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