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Evaluation of the safety and quality of Brazil nuts () using the tools of dna sequencing technology and aflatoxin profile.

Front Nutr

April 2024

Embrapa Food Technology, Office of Research and Development (Sector: Operational Units - Plan V), Rio de Janeiro, Brazil.

Introduction: Brazil nuts (BNs) result from sustainable extraction and are widely exploited in the Amazon region. Due to the production characteristics in the forest and the nutritional characteristics of these nuts, the occurrence of fungal contamination and the presence of aflatoxins are extensively discussed in the literature as a great aspect of interest and concern. This study aims to evaluate the microbial profile through DNA sequencing and amplification of 16S and ITS genes for bacterial and fungal analysis, respectively, and the presence of mycotoxins using high-performance liquid chromatography with fluorescence detection (HPLC-FD) from different fractions of the nuts processed.

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Objective: Introduction: In the course of the study, we identified high prevalence rates of orthodontic pathology and identified organizational aspects regarding the provision of orthodontic care for children, and identified the need for their optimization. The aim: The purpose of the study was to analyze the results of the questionnaire of orthodontists and children's dentists, who provide dental care to the children of the Poltava region, about the importance of optimization of orthodontic care for children and improving the knowledge of doctors about the risk factors for the onset of orthodontic pathology.

Patients And Methods: Materials and methods: Methods: Sociological (questionnaire), medical-statistical, bibliosemantic.

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An analysis of 2848 cases of complicated purulent diseases of upper extremities has determined the main errors of the prehospital treatment of the infection. Irrational self-reliant and ambulatory treatment resulted in the appearance of marked functional and anatomical defects of the affected extremity which lead to considerable economical costs. The following principal ways to solve the found problems were established: sanitary-educational work among the population, training of the ambulatory link of specialists to learn modern aspects of the diagnosis and treatment of purulent diseases of upper extremities.

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The implementation of regional tuberculosis control programs contributes to the improvement of the tuberculosis epidemic situation. The holding of a stimulating lottery among those who have undergone fluorographic study is a novel approach that increases the coverage of particularly the unorganized population with preventive fluographic studies. Social support of patients with tuberculosis improves the results of their treatment.

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