The study was aimed at evaluation of usefulness of PYR-Wellcome test in bacteriological diagnostic. Results of identification of enterococcal strains by application of the above test were performed on 157 strains of Enterococcus spp., 15 strains of Streptococcus pyogenes and 12 of Streptococcus bovis. Application of test for PYR-ase production results in shortening of diagnostic procedure. Basing on morphological features, including type of hemolysis, lack of catalase production and presence in the bacterial cell of a peptidase hydrolyzing L-pirrolidonyl-beta-naphthylamide, it is possible to classify with high probability the investigated microorganism to the Enterococcus spp. The Shortened procedure is satisfactory for routine identification of enterococcal strains in diagnostic laboratories.

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