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Comparison of three computer methods of sperm head analysis. | LitMetric
Objective : Analysis of sperm heads using three different computer morphometrical tools and experimental conditions to find a more reliable and secure strategy among them.Design : Controlled experiments on sperm morphology analysis from volunteers.Setting : Laboratory of microscopy and imaging processing.Patient(s) : Ten human semen samples donated by different zoospermic men.Intervention(s) : Semen samples were collected by masturbation after > or =72 hours of abstinence.Main Outcome Measure(s) : Spermatozoon head morphology was compared by the use of different video-microscopy systems, three computer programs, and various staining conditions and manipulation by different operators. Nonbiological material in the form of latex beads was also used.Result(s) : The data obtained suggest that the semiautomatic computer program is the most reliable and secure method for performing sperm analysis, besides the fact that it is a fast process compared with manual methods.Conclusion(s) : Computer systems of sperm analysis should incorporate a step of interactive object identification to work properly, allowing the operator to confirm or correct possible computer misidentification. The latex beads were used to confirm the capability of all three computer programs to correctly evaluate nonbiological material.
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