AI Article Synopsis
- A study compared PCR testing with culture methods for diagnosing Mycoplasma intramammary infection in cows, focusing on milk samples.
- The PCR test showed high sensitivity (96.2% for individual cow milk and 100% for bulk tank milk) and specificity (99.1% and 99.8%, respectively).
- In cases where PCR results conflicted with culture results, PCR proved accurate, and it could identify 11 different Mycoplasma species at once.
Article Abstract
A polymerase chain reaction (PCR) test was compared with culture for the detection and diagnosis of bovine Mycoplasma intramammary infection. The PCR test was applied to 24-hour Mycoplasma enrichment cultures of milk from cows with suspected mastitis and from bulk tank milk. In comparison to culture, the sensitivity and specificity of the PCR method were 96.2% and 99.1% for individual cow milk and 100% and 99.8% for the bulk tank milk, respectively. However, in discrepant cases where PCR was positive and culture was negative, the PCR test was correct; subsequent PCR tests and culturing of the individual cow's milk yielded positive results. The PCR test simultaneously detected and differentiated among 11 bovine Mycoplasma species.
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| http://dx.doi.org/10.1177/104063879901100507 | DOI Listing |
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