Purpose: To report confocal microscopic observation of rabbit corneal epithelium through amniotic membrane patch (AMP).
Design: Experimental study.
Method: Six rabbit eyes were patched by amniotic membrane. Then, white-light and laser confocal microscopic observation was performed.
Results: Images of human amniotic epithelium, amniotic basement membrane, and amniotic stroma were obtained using both devices, followed by detection of rabbit corneal epithelium.
Conclusion: This study indicates that observation of corneal epithelium through AMP is possible in rabbit eyes using both these methods. This implies that both devices might be used clinically to observe the epithelial healing process under an AMP to better determine when it should be removed.
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http://dx.doi.org/10.1016/s0002-9394(03)00325-8 | DOI Listing |
Chem Asian J
December 2024
Indian Institute of Technology Ropar, Department of Chemistry, Bara Phool, 140001, Rupnagar, INDIA.
Ocular drug-delivery is one of the most challenging areas owing to nature of ocular tissues. Various nanoformulations have been designed and investigated for drug-delivery to achieve high drug bioavailability. The major focus of preparations available in market is to utilize nanomaterial as drug-carrier, with less focus on developing functional-nanomaterials, which is a key knowledge gap in the field.
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December 2024
Harry S. Truman Memorial Veterans' Hospital, Columbia, MO, United States; Departments of Veterinary Medicine & Surgery and Biomedical Sciences, University of Missouri, Columbia, MO, United States; Mason Eye Institute, School of Medicine, University of Missouri, Columbia, MO, United States. Electronic address:
Sulfur mustard gas (SM), an alkylating and vesicating agent, has been used frequently in many wars and conflicts. SM exposure to the eye results in several corneal abnormalities including scar/fibrosis formation. However, molecular mechanism for SM induced corneal fibrosis development is poorly understood.
View Article and Find Full Text PDFSci Rep
December 2024
Department of Medical Application of Laser, Ophthalmic Unit, National Institute of Laser Enhanced Sciences, Cairo University, Giza, Egypt.
Corneal alkali burns persist as a significant challenge in our field, often leading to a prolonged treatment course with various sight-threatening problems. This work, of utmost importance, aimed to apply the photo-tissue bonding technique (PTB) to weld the amniotic membrane (AM) to the corneal surface versus an amniotic membrane graft (AMG) and explore its safety in saving corneal protein against alkali burn.Methods Twenty-seven rabbits with an induced corneal ulcer using 1 mol/L NaOH solution.
View Article and Find Full Text PDFSci Adv
December 2024
Eye Center, the Second Affiliated Hospital, School of Medicine, Zhejiang University, Zhejiang Provincial Key Laboratory of Ophthalmology, Zhejiang Provincial Clinical Research Center for Eye Diseases, Zhejiang Provincial Engineering Institute on Eye Diseases, Hangzhou 310009, P. R. China.
Corneal injury-induced fibrosis occurs because of corneal epithelial basement membrane (EBM) injury and defective regeneration. Corneal fibrosis inhibition and transparency restoration depend on reestablished EBM, where the collagen network provides structural stability and heparan sulfate binds corneal epithelium-derived cytokines to regulate homeostasis. Inspired by this, bioactive hydrogels (Hep@Gel) composed of collagen-derived gelatins and highly anionic heparin were constructed for scarless corneal repair.
View Article and Find Full Text PDFSmall
December 2024
National Engineering Research Center for Biomaterials, College of Biomedical Engineering, Sichuan University, Chengdu, Sichuan, 610065, China.
Collagen as the main structural component of the cornea exhibits unique and highly organized fibril lamellae, which contribute to the maintenance of corneal structure and transparency. Nevertheless, collagen assembly in vitro to create ideal artificial corneal substitutes with human cornea comparable thickness and optics is still limited. Here, glycerol as a regulator can reconcile collagen thickness, transparency, and permeability, a conflicting goal by current keratoprosthesis strategies.
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