Role of nitric oxide in apoptosis and cell necrosis for intestinal transplantation.

Transplant Proc

Department of Surgery-Campus Bellvitge, University of Barcelona, Barcelona, Spain.

Published: August 2003

AI Article Synopsis

  • Nitric oxide (NO) plays a complex role in mediating responses during ischemia-reperfusion syndrome, impacting cell death processes like apoptosis and necrosis.
  • In a study with rats undergoing small bowel transplants, different groups were treated with NO donors, NO inhibitors, or left as controls to analyze their effects on cellular damage.
  • Results indicated that exogenous NO reduced necrosis of the transplants but simultaneously increased apoptosis, suggesting a dual effect that warrants further investigation.

Article Abstract

Introduction: Nitric oxide (NO) is an important mediator of both physiological and pathological responses. Its dual role in the ischemia-reperfusion syndrome is still a matter of controversy. The aim of this study was to analyze the effect of NO on apoptosis and cell necrosis associated with heterotopic small bowel transplant.

Methods: Sprague-Dawley rats underwent heterotopic small bowel transplants with 3 hours of cold ischemia and 5 hours of reperfusion. Animals were assigned to the following study groups: Sham; bowel transplant (Trp); bowel transplant + NO donor (Trp + NONOS); bowel transplant + NO synthesis inhibitor (Trp + L-NAME). We studied histological changes and bacterial translocation in mesenteric nodes, liver and spleen as parameters of cell necrosis and caspase-3 activity as a parameter of apoptosis.

Results: Histological changes and bacterial translocation showed that exogenous administration of NO protected the transplant. Simple bowel transplant, with or without inhibition of NO synthesis, did not display this protective effect. Significantly greater levels of apoptosis were observe in grafts among the group administered NO at pharmacological doses.

Conclusions: In experimental bowel transplantation rats administered exogenous NO show less necrosis but at the same time stimulation of apoptosis.

Download full-text PDF

Source
http://dx.doi.org/10.1016/s0041-1345(03)00729-2DOI Listing

Publication Analysis

Top Keywords

bowel transplant
16
cell necrosis
12
nitric oxide
8
apoptosis cell
8
heterotopic small
8
small bowel
8
histological changes
8
changes bacterial
8
bacterial translocation
8
bowel
7

Similar Publications

The identification of immune environments and cellular interactions in the colon microenvironment is essential for understanding the mechanisms of chronic inflammatory disease. Despite occurring in the same organ, there is a significant gap in understanding the pathophysiology of ulcerative colitis (UC) and colorectal cancer (CRC). Our study aims to address the distinct immunopathological response of UC and CRC.

View Article and Find Full Text PDF

The role of the immune system in regulating tissue stem cells remains poorly understood, as does the relationship between immune-mediated tissue damage and regeneration. Graft vs. host disease (GVHD) occurring after allogeneic bone marrow transplantation (allo-BMT) involves immune-mediated damage to the intestinal epithelium and its stem cell compartment.

View Article and Find Full Text PDF

This study aimed to analyze the homology between carbapenem-resistant organisms (CRO) intestinal colonization strains and bloodstream infection (BSI) strains in patients undergoing hematopoietic stem cell transplantation (HSCT), confirming the clinical use of the real-time rectal swab Xpert Carba-R assay, and investigate its feasibility in early warning of BSI. Drug-resistant strains obtained from rectal swabs and blood culture samples of patients undergoing the same HSCT from January 2021 to December 2021 were collected and analyzed. The homology of the CRO intestinal colonization and BSI strains was confirmed using strain identification, antimicrobial resistance phenotyping, whole genome sequencing (WGS), multilocus sequence typing (MLST), and carbapenemase type identification.

View Article and Find Full Text PDF

Background/aims: Optimization of bowel preparation for small bowel capsule endoscopy (SBCE) is debated. Guidelines recommend 2 L of iso-osmolar polyethylene glycol (PEG) to improve SBCE visibility. We compared the efficacy of the standard 2 L PEG solution with a 1 L PEG plus ascorbate (PEG-ASC) preparation, which has already been established for large-bowel preparation.

View Article and Find Full Text PDF

Are enteral devices risk factors for central line-associated bloodstream infections in children with intestinal failure?

Clin Nutr

December 2024

Institute of Health Policy, Management and Evaluation, University of Toronto, Toronto, Ontario, Canada; Division of General and Thoracic Surgery, Cincinnati Children's Hospital Medical Center, University of Cincinnati, Cincinnati, OH, USA; Cincinnati Center of Excellence for Intestinal Rehabilitation (CinCEIR), Cincinnati Children's Hospital Medical Center, Cincinnati, OH, USA. Electronic address:

Background & Aims: Central line-associated bloodstream infections (CLABSI) represent one of the most common and serious complications in children with intestinal failure (IF). This study aimed to assess if there is an association between the use of enteral devices (feeding tubes and stomas) with rate of CLABSI after adjusting for clinically relevant factors. Second, association between enteral devices with time to first CLABSI event was evaluated.

View Article and Find Full Text PDF

Want AI Summaries of new PubMed Abstracts delivered to your In-box?

Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!