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From signal-off to signal-on: polyT linker alters signal response mode and enhances signal change of aptamer beacon probe.
Anal Bioanal Chem
January 2025
College of Chemistry and Chemical Engineering, Linyi University, Linyi, 276000, China.
A molecular beacon is an oligonucleotide hybridization probe that can report the presence of specific nucleic acids in homogeneous solutions. Using an aptamer has allowed an aptamer-based molecular beacon-aptamer beacon to be developed, which has shown advantages of simplicity, rapidity, and sensitivity in imaging and sensing non-nucleic acid substances. However, due to requirement for a deliberate DNA hairpin structure for the preparation of a molecular beacon, not any given aptamer is suitable for designing an aptamer beacon probe.
View Article and Find Full Text PDFMultimodal targeting chimeras enable integrated immunotherapy leveraging tumor-immune microenvironment.
Cell
December 2024
Synthetic and Functional Biomolecules Center, Beijing National Laboratory for Molecular Sciences, Key Laboratory of Bioorganic Chemistry and Molecular Engineering of Ministry of Education, College of Chemistry and Molecular Engineering, Peking University, Beijing 100871, China; Shenzhen Bay Laboratory, Shenzhen 518055, China; Peking-Tsinghua Center for Life Sciences, Peking University, Beijing 100871, China. Electronic address:
Although immunotherapy has revolutionized cancer treatment, its efficacy is affected by multiple factors, particularly those derived from the complexity and heterogeneity of the tumor-immune microenvironment (TIME). Strategies that simultaneously and synergistically engage multiple immune cells in TIME remain highly desirable but challenging. Herein, we report a multimodal and programmable platform that enables the integration of multiple therapeutic modules into single agents for tumor-targeted co-engagement of multiple immune cells within TIME.
View Article and Find Full Text PDFNovel Vanillin-based hybrids inhibit quorum sensing and silences phenotypical expressions in Pseudomonas aeruginosa.
Drug Dev Res
February 2023
Department of Applied Sciences, Punjab Engineering College (Deemed to be University), Chandigarh, India.
In this study, we report the chemical synthesis, computational analysis, and anti-virulent studies of five Vanillin-based hybrids employing phytochemicals. Vanillin (V) is known to have substantial anti-quorum sensing activity against the gram-negative pathogen Pseudomonas aeruginosa. Therefore, with the aim to further enhance the potency of Vanillin, it was chemically conjugated via a triazole (T) linker with five phytochemicals- Zingerone (Z), Eugenol (E), Guaiacol (G), Cinnamaldehyde (C), and Ferulic acid (F) to form the hybrids named as VTZ (1), VTE (2), VTG (3), VTC (4), and VTF (5), respectively.
View Article and Find Full Text PDFMulti-code magnetic beads based on DNAzyme-mediated double-cycling amplification for a point-of-care assay of telomerase activity.
Analyst
July 2019
Department of Chemistry, Liaocheng University, Liaocheng, 252059, Shandong, China.
Development of a reliable and facile telomerase activity assay with high specificity and sensitivity is a central challenge to make telomerase testing a routine part of medical care with respect to cancer. Herein, we propose a point-of-care (POC) assay of telomerase activity based on multi-code magnetic beads initiated by DNAzyme-mediated double-cycling amplification coupling with a glucometer. A designed DNAzyme-based telomerase substrate prime (DTSP) probe consists of three regions (AXB) for a DNAzyme catalytic sequence, poly-thymine (poly-T) linker and telomere primer sequence.
View Article and Find Full Text PDFA-T linker adapter polymerase chain reaction for determining flanking sequences by rescuing inverse PCR or thermal asymmetric interlaced PCR products.
Anal Biochem
December 2014
Laboratory of Food Safety, College of Food Science and Nutritional Engineering, China Agricultural University, Beijing 100083, China; Supervision, Inspection, and Testing Center of Genetically Modified Organisms, Ministry of Agriculture, Beijing 100083, China.
The polymerase chain reaction (PCR)-based genome walking method has been extensively used to isolate unknown flanking sequences, whereas nonspecific products are always inevitable. To resolve these problems, we developed a new strategy to isolate the unknown flanking sequences by combining A-T linker adapter PCR with inverse PCR (I-PCR) or thermal asymmetric interlaced PCR (TAIL-PCR). The result showed that this method can be efficiently achieved with the flanking sequence from the Arabidopsis mutant and papain gene.
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