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An ultrasensitive colorimetric/fluorescent/photothermal sensing platform for the detection of D-amino acids based on carbon dots nanozymes with enhanced peroxidase-like activity.
Mikrochim Acta
January 2025
Department of Chemistry, School of Science, Xihua University, Chengdu, 610039, PR China.
Based on the enhanced peroxidase-like activity of carbon dots nanozymes (CDszymes), with a specific oxidation reaction of D-amino acid oxidase catalysing the formation of HO from D-amino acid, an ultrasensitive sensing platform, was constructed for the quantitative detection of D-amino acids in saliva. With the increase of D-amino acids concentration, the blue color of catalytic product gradually deepend, the fluorescence CDszymes gradually quenched, and the temperature gradually increased. Using D-alanine as D-amino acid models, the detection limits of D-alanine in colorimetric/photothermal/fluorescent mode were 0.
View Article and Find Full Text PDFAn "on-off" electrochemical immunosensor for the detection of the glycan antigen CA125 by amplification signals using electropositive COFs.
Talanta
January 2025
College of Chemistry and Materials, Jiangxi Normal University, 99 Ziyang Avenue, Nanchang, 330022, China.
Cancer Antigen 125 (CA125), is a high molecular weight mucinous glycoprotein found on the surface of ovarian cancer cells. Generally, 90 % of women may appear a high concentration of CA125 when they got the cancer; thus, CA125 can act as a marker for ovarian cancer diagnosis and therapeutic evaluation. COFs have been widely used for disease detection due to their structural stability, high loading capacity and biocompatibility.
View Article and Find Full Text PDFA Prototype of Ultrasensitive Time-Resolved Fluoroimmunoassay with Enhanced Fluorescence System for the Trace Determination of Urinary 8-Hydroxy-2`-Deoxyguanosine, the DNA Oxidative Stress Biomarker.
J Fluoresc
January 2025
Department of Chemistry, Faculty of Science, Chulalongkorn University, Bangkok, Phayathai Road Pathumwan, 10330, Thailand.
This study presents a new highly sensitive and specific time-resolved fluoroimmunoassay (TRFIA) for the measurement of trace amounts of the urinary 8-hydroxy-2`-deoxyguanosine (8-OHdG) which is a biomarker for oxidative stress on DNA. The assay relied on a competitive binding approach and a mouse monoclonal antibody which recognized 8-OHdG with high specificity. In this assay, 8-OHdG conjugated with bovine serum albumin protein (8-OHdG-BSA) was employed as a solid phase antigen.
View Article and Find Full Text PDFUltrasensitive "On-Off" Ratiometric Fluorescence Biosensor Based on RPA-CRISPR/Cas12a for Detection of .
J Agric Food Chem
January 2025
School of Food and Biological Engineering, Jiangsu University, Zhenjiang 212013, China.
() is a major pathogenic bacterium responsible for bacterial foodborne diseases, making its rapid, specific, and accurate detection crucial. In this study, we develop a ratiometric biosensor based on the recombinase polymerase amplification-clustered regularly interspaced short palindromic repeats/CRISPR associated protein 12a (RPA-CRISPR/Cas12a) system and Eu-metal-organic framework (Eu-MOF) fluorescent nanomaterials for the high-sensitivity detection of , combining with RPA for efficient isothermal amplification, this sensor enhances specificity and sensitivity by utilizing the target activation of CRISPR/Cas12a. The Eu-MOF serves a dual function, providing stable red fluorescence as a reference signal and adsorbing FAM-labeled probes for fluorescence quenching, forming a dual-signal system that significantly reduces background interference.
View Article and Find Full Text PDFA simply synthesized, silver ions-doped porous gold microparticles-based SERS aptamer sensor for ultrasensitive and broad-range quantitative detection of IL-6.
Anal Chim Acta
January 2025
Key Laboratory of Biomaterials of Guangdong Higher Education Institutes, Department of Biomedical Engineering, Jinan University, Guangzhou, 510632, China. Electronic address:
Background: The multifunctional cytokine interleukin-6 (IL-6) plays a pivotal role in chronic and acute inflammatory responses, underscoring the importance of accurately determining IL-6 levels for early diagnosis, prevention, and treatment of inflammation.
Results: This study developed a versatile and innovative single-particle surface-enhanced Raman spectroscopy (SERS) sensing platform for the precise and sensitive quantification of IL-6 in complex samples using a novel one-pot synthesized, silver ions-doped three-dimensional porous gold microparticles (PGMs) with abundant hot spots for robust SERS enhancement. By rationally designing rich cytosine-Ag-cytosine base pairs between IL-6 aptamers and complementary chains on the PGMs, we harnessed the SERS-enhancing effect to achieve highly sensitive and specific IL-6 quantification within a wide range of 10 to 10 mg/mL and a limit of detection (LOD) of 0.
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