A chemically defined medium that supports the growth of both encapsulated and nontypeable Haemophilus influenzae strains in broth to densities that are >/= 10(9) CFU/ml or on agar plates is described. The mean generation time of a panel of clinical isolates was comparable to that in rich, chemically undefined media (brain-heart infusion broth supplemented with heme and beta-NAD).

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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC193788PMC
http://dx.doi.org/10.1128/JCM.41.9.4408-4410.2003DOI Listing

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