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METTL7A improves bovine IVF embryo competence by attenuating oxidative stress.
Biol Reprod
January 2025
Department of Animal Sciences, Genetics Institute, University of Florida, Gainesville, FL, USA.
In vitro fertilization (IVF) is a widely used assisted reproductive technology to achieve a successful pregnancy. However, the acquisition of oxidative stress in embryo in vitro culture impairs its competence. Here, we demonstrated that a nuclear coding gene, methyltransferase-like protein 7A (METTL7A), improves the developmental potential of bovine embryos.
View Article and Find Full Text PDFBioengineering Approaches for Male Infertility: From Microenvironmental Regeneration to in vitro Fertilization.
Adv Exp Med Biol
January 2025
METU MEMS Center, Ankara, Turkey.
Male factor accounts for 30-50% of infertility cases and may occur due to congenital anomalies or acquired disorders. In such infertility cases where a limited number of mature sperm is produced, a solution is offered to patients with ART applications; however, these methods are inadequate in patients with germ cell aplasia due to damaged microenvironment. Since monolayer cell culture and static culture conditions do not provide the physical conditions of the 3D microenvironment, they have a limited effect on ensuring the execution of in vitro spermatogenesis properly.
View Article and Find Full Text PDFEffect of Time-Lapse Incubation System on In Vitro Development of Alpaca Embryos.
Reprod Domest Anim
February 2025
Veterinary Embryology Laboratory, Professional School of Veterinary Medicine, Universidad Nacional de San Antonio Abad del Cusco, Sicuani-Cusco, Peru.
Currently, incubators with a time-lapse system are widely used for in vitro embryo production in several species, however, their effect on alpaca embryo development compared to conventional incubators remains unknown. The aim of this study was to compare early in vitro embryo development in alpacas using a time-lapse incubator system versus a conventional incubator. Ovaries were obtained from a slaughterhouse and 1048 cumulus-oocyte complexes (COCs) were collected and in vitro matured for 26 h in either a time-lapse system (n = 542) or a conventional incubator (n = 542).
View Article and Find Full Text PDFLack of effect of cyclic adenosine monophosphate modulation during prematuration in vitro on development to the blastocyst stage and expression of specific genes associated with lineage commitment.
JDS Commun
January 2025
Department of Animal Sciences and D.H. Barron Reproductive and Perinatal Biology Research Program, University of Florida, Gainesville, FL 32611-0910.
Pharmacological elevation of cyclic AMP (cAMP) of cultured cumulus-oocyte complexes (COC) before or coincident with initiation of maturation has been reported to improve outcomes for various systems for in vitro production of embryos. Here it was hypothesized that artificial elevation of cAMP in the oocyte for a 2-h period of prematuration would improve developmental competence of matured oocytes and result in increased blastocyst yield and altered expression of genes important for embryonic differentiation. Treated COC were cultured for 2 h with dibutyryl cAMP (dbcAMP), a membrane-permeable form of cAMP, and 3-isobutyl-1-methylxanthine (IBMX), which inhibits phosphodiesterases that convert cAMP to ATP.
View Article and Find Full Text PDFActivation of Proteolysis During Oocyte In Vitro Maturation.
Mol Reprod Dev
January 2025
Liv Hospital, Centre for Regenerative Medicine and Stem Cell Manufacturing (LivMedCell), İstanbul, Turkey.
In vitro maturation (IVM) is a form of assisted reproductive technology (ART) applied to obtain mature oocytes in culture. Decline in IVM success rates by age has led consideration of novel approaches based on cellular dynamics. Our aim was to achieve proteostasis in old bovine oocytes from 13 to 16-year-old bovine with a lower potential for fertilization.
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