Analysis of the indicator synthesis was studied by catalytic spectrophotometry, and the reducing decolorization that the copper(II) catalyzed the ration between ascorbic acid and 3-methyl-4-amino-4'-nitro azobenzol was discussed. Reaction condition was optimized and a highly selective determination of trace copper(II) was established. The detection limit was 0.048 microgram.L-1 for copper (II). The method has been used for determining trace copper(II) in human hair and aluminum alloy samples with satisfactory results.
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