[Study on quantitative assay of chondroitin sulfate with a spectrophotometric method of azure A].

Guang Pu Xue Yu Guang Pu Fen Xi

Pharmaceutic Biotechnology Key Laboratory, College of Life Science, Nanjing University, Nanjing 210093, China.

Published: June 2003

A simple, accurate and rapid spectrophotmeric method was proposed for the determination of chondroitin sulfate. The method was based on the absorbances of AA-CS complex at 625 nm being in proportion to the chondroitin sulfate concentrations. The linear range was 0-30 micrograms.mL-1 (R = 0.999), and the recovery range was 97.4%-103.8%. The quantities of chondroitin sulfate in different sample were determined in this way.

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