AI Article Synopsis
- Researchers studied a UV light-sensitive mutant called rev3-1 in the plant Arabidopsis, which showed impaired root growth after UV-B exposure.
- Chromosome analysis revealed that rev3-1 had multiple breaks leading to chromosome inversions and translocations, disrupting a gene linked to DNA polymerase zeta (AtREV3) and affecting DNA repair.
- The rev3-1 mutant also showed heightened sensitivity to gamma-rays and mitomycin C, with reduced DNA replication following UV-B damage, which inhibited cell division and root growth.
Article Abstract
To investigate UV light response mechanisms in higher plants, we isolated a UV light-sensitive mutant, rev3-1, in Arabidopsis. The root growth of rev3-1 was inhibited after UV-B irradiation under both light and dark conditions. We found that chromosome 1 of rev3-1 was broken at a minimum of three points, causing chromosome inversion and translocation. A gene disrupted by this rearrangement encoded the catalytic subunit of DNA polymerase zeta (AtREV3), which is thought to be involved in translesion synthesis. The rev3-1 seedlings also were sensitive to gamma-rays and mitomycin C, which are known to inhibit DNA replication. Incorporation of bromodeoxyuridine after UV-B irradiation was less in rev3-1 than in the wild type. These results indicate that UV light-damaged DNA interrupted DNA replication in the rev3-1 mutant, leading to the inhibition of cell division and root elongation.
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| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC181330 | PMC |
| http://dx.doi.org/10.1105/tpc.012369 | DOI Listing |
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