Isolated hepatocytes are known to maintain their physiological functions for over a week when cultured on Matrigel, artificially reconstituted from basement membrane components. Although this culture technique has been frequently used in research on hepatocyte functions, there has been a limitation on its application for small scale experiments due to some technical problems. By using micro-culture plates with 96 round-bottom wells, we succeeded in coating the wells uniformly with Matrigel. When the cultured hepatocytes were treated with either 10 mM, 15 mM, or 20 mM of acetaminophen or 1 mM, 10 mM, or 20 mM of D-galactosamine, the viability of the hepatocytes became 91.1%, 75.3%, 64.7%, and 79.0%, 43.8%, 26.2% of the non-treated control at 48 hours, respectively. Fractionated extracts of Glycyrrhiza glabra L. and Schisandra chinensis Baillon inhibited the action of acetaminophen or D-galactosamine in this model. From these results, we concluded that the microculture system presented here is capable of maintaining the in vivo characteristics of hepatocytes and is suitable for the screening of hepatoprotective substances.
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http://dx.doi.org/10.1271/bbb.67.1629 | DOI Listing |
Sci Rep
January 2025
Institute of the Malay World and Civilisation (ATMA), Universiti Kebangsaan Malaysia (UKM), 43600, Bangi, Selangor, Malaysia.
The adoption of pultruded glass fibre-reinforced polymer (pGFRP) composites as a substitute for traditional wooden cross-arms in high transmission towers represents a relatively novel approach. These materials were selected for their high strength-to-weight ratio and lightweight properties. Despite various studies focusing on structures improvement, there still have a significant gap in understanding the deformation characteristics of full-scale cross-arms under actual operational loads.
View Article and Find Full Text PDFNat Commun
January 2025
Data Science Institute, Imperial College London, London, UK.
AI techniques are increasingly being used to identify individuals both offline and online. However, quantifying their effectiveness at scale and, by extension, the risks they pose remains a significant challenge. Here, we propose a two-parameter Bayesian model for exact matching techniques and derive an analytical expression for correctness (κ), the fraction of people accurately identified in a population.
View Article and Find Full Text PDFNat Commun
January 2025
Chair for Bioinformatics, Institute for Computer Science, Friedrich Schiller University Jena, Jena, Germany.
Small molecule machine learning aims to predict chemical, biochemical, or biological properties from molecular structures, with applications such as toxicity prediction, ligand binding, and pharmacokinetics. A recent trend is developing end-to-end models that avoid explicit domain knowledge. These models assume no coverage bias in training and evaluation data, meaning the data are representative of the true distribution.
View Article and Find Full Text PDFJ Voice
January 2025
Utah Center for Vocology, University of Utah, Salt Lake City, UT; National Center for Voice and Speech, Salt Lake City, UT. Electronic address:
Objectives: Acoustic and aerodynamic powers in infant cry are not scaled downward with body size or vocal tract size. The objective here was to show that high lung pressures and impedance matching are used to produce power levels comparable to those in adults.
Study Design And Methodology: A computational model was used to obtain power distributions along the infant airway.
BMJ Open
January 2025
School of Clinical Medicine, Faculty of Medicine and Health, University of New South Wales, Sydney, New South Wales, Australia
Objective: To evaluate the relationship between infarct pattern, inferred stroke mechanism and risk of recurrence in patients with ischaemic stroke. The question is clinically relevant to optimise secondary stroke prevention investigations and treatment.
Design: We conducted a retrospective analysis of the dabigatran treatment of acute stroke II (DATAS II) trial (ClinicalTrials.
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