AI Article Synopsis
- The study examined the genetic diversity of 19 Porphyromonas gingivalis isolates from periodontitis patients using multilocus sequence analysis.
- Researchers amplified and sequenced DNA fragments from 10 chromosomal genes, finding that no two isolates were identical at these loci.
- Phylogenetic analysis revealed a panmictic population structure and highlighted the significant role of recombination in the genetic variation of P. gingivalis, with an index of association suggesting the genes are close to linkage equilibrium.
Article Abstract
In this study, the genetic relationship of 19 Porphyromonas gingivalis isolates from patients with periodontitis was investigated by multilocus sequence analysis. Internal 400-600 bp DNA fragments of the 10 chromosomal genes ef-tu, ftsQ, hagB, gpdxJ, pepO, mcmA, dnaK, recA, pga and nah were amplified by PCR and sequenced. No two isolates were identical at all 10 loci. Phylogenetic analyses indicated a panmictic population structure of P. gingivalis. Split decomposition analysis, calculation of homoplasy ratios and analyses of clustered polymorphisms all indicate that recombination plays a major role in creating the genetic heterogeneity of P. gingivalis. A standardized index of association of 0.0898 indicates that the P. gingivalis genes analysed are close to linkage equilibrium.
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| http://dx.doi.org/10.1099/mic.0.26267-0 | DOI Listing |
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