A novel analytical approach based on a combination of multidimensional hyphenated techniques and cloning of the Ni-resistance gene using yeast complementation screens was developed for the identification of nickel species in a Thlaspi caerulescens hyperaccumulating plant. The presence of an unknown strong Ni complex was demonstrated by size exclusion HPLC-capillary electrophoresis with ICPMS detection. The Ni-containing peak was characterized by electrospray MS (m/z 360) and shown by collision-induced dissociation MS to be a chelate with a tricarboxylic amino acid ligand. To identify the species and demonstrate its functional character, a cDNA library was constructed from T. caerulescens, expressed in the yeast, and screened on a toxic Ni2+ medium. The extract from the surviving transformant culture gave identical HPLC-ICPMS, CZE-ICPMS, and ES MS/MS data and contained a cDNA insert homologous to the nicotianamine synthase gene. This observation allowed the identification of nicotianamine as the nickel-binding ligand. The presence of the Ni-nicotianamine complex was ultimately demonstrated by comparing tandem mass spectra of the plant and yeast extracts with those of a synthetic standard.
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http://dx.doi.org/10.1021/ac020704m | DOI Listing |
Ecotoxicol Environ Saf
January 2025
Department of Biosystems and Technology, Swedish University of Agricultural Sciences, Alnarp 23456, Sweden. Electronic address:
Nickel (Ni) is required in trace amounts (less than 500 µg kg) in plants to regulate metabolic processes, the immune system, and to act as an enzymatic catalytic cofactor. Conversely, when nickel is present in high concentration, it is considered as a toxic substance. Excessive human nickel exposure occurs through ingestion, inhalation, and skin contact, ultimately leading to respiratory, cardiovascular, and chronic kidney diseases.
View Article and Find Full Text PDFAnticancer Agents Med Chem
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Department of Biotechnology, Jamia Hamdard, New Delhi, 110062, India.
This review investigates the outcome of heavy metal contamination on the anti-cancer properties of medicinal plants. Heavy metal pollution is a significant environmental concern globally, often found in soil and water due to industrial activities. Therapeutic plants are recognized because of their therapeutic attributes and their ability to absorbing these contaminants.
View Article and Find Full Text PDFToxics
November 2024
Sede Vallenar, Universidad de Atacama, Av. Costanera #105, Vallenar 1612178, Chile.
Copper mining drives economic growth, with the global demand expected to reach 120 million metric tons annually by 2050. However, mining produces tailings containing heavy metals (HMs), which poses environmental risks. This study investigated the efficacy of phytoremediation (Phy) combined with electrokinetic treatment (EKT) to increase metal uptake in grown in tailings from the Metropolitan Region of Chile.
View Article and Find Full Text PDFACS Appl Mater Interfaces
January 2025
School of Chemistry and Materials Science, Ludong University, Yantai 264025, China.
The cadmium (Cd) in saline-alkali soil poses a serious threat to the ecological environment and human health. , as a hyperaccumulator plant, can remediate Cd in saline-alkali soil, but the efficiency of phytoremediation is low. To improve the remediation effect of Cd pollution in saline-alkali soil, this study for the first time uses the synergy of hydrogel and for the remediation of Cd in saline-alkali soil.
View Article and Find Full Text PDFJ Environ Manage
January 2025
Department of Earth and Environmental Sciences, National Chung Cheng University, Chiayi County, Taiwan. Electronic address:
Serpentine soils are characterized by high concentrations of heavy metals (HMs) and limited essential nutrients with remarkable endemic plant diversity, yet the mechanisms enabling plant adaptation to thrive in such harsh environments remain largely unknown. Full-length 16S rRNA amplicon sequencing, coupled with physiological and functional assays, was used to explore root-associated bacterial community composition and their metabolic and ecological functions. The results revealed that serpentine plant species exhibited significantly higher metal transfer factor values compared to non-serpentine plant species, particularly evident in Bidens pilosa, Miscanthus floridulus, and Leucaena leucocephala.
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