AI Article Synopsis
- The study aimed to set up a reliable culture system for mouse embryonic fibroblasts (MEF).
- MEF were isolated from BALB/c mouse embryos, with optimal isolation occurring at 13.5 days of gestation, and found to have good growth properties by passage 3.
- Results indicate that MEFs (passage 3) are ideal for use as a feeder layer for embryonic stem cell cultures.
Article Abstract
Objective: To establish a stable culture system of mouse embryonic fibroblast(MEF).
Methods: We isolated MEF from the embryos of BALB/c mouse and investigated the morphology, growth curve and cell cycle of MEF in vitro. The effects of the gestational age (days) of mouse embryos and the time of digestion on the isolation and culture of MEF were assessed.
Results: The mouse embryo of 13.5 d is better than that of 10.5 d and 18.5 d on the isolation of MEF. MEF in vitro is a kind of adherent cell with good ability for proliferation in passage 3. In room temperature, the digestive time of 0.25% trypsin should be 3-5 min.
Conclusion: MEFs(P3) are quite suited to be a feeder layer of embryonic stem cells.
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