Electric charge distribution in mRNA 5' cap terminus has been exhaustively characterized in respect to the affinity for cap-binding proteins. Formation of the stacked configuration of positively charged 7-methylguanine in between two aromatic amino acid rings, known as sandwich cation-pi stacking, is thought to be prerequisite for the specific recognition of the cap by eukaryotic initiation factor eIF4E; i.e., discrimination between the cap and nucleotides without the methyl group at N(7). Nuclear magnetic resonance spectroscopy of (15)N/(13)C-double-labeled 7-methylguanosine 5'-triphosphate and 7-methylguanosine, as well as their unsubstituted counterparts, GTP and guanosine, yielded characteristic changes of the electron-mediated spin-spin couplings and chemical shifts due to the methylation at N(7). The experimentally measured changes of the nuclear magnetic resonance parameters have been analyzed in respect to the electric charge distribution calculated by means of quantum chemical methods, and interpreted in terms of new proposed positive charge localization in the 7-methylguanine five-member ring.
Download full-text PDF |
Source |
|---|---|
| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC1303321 | PMC |
| http://dx.doi.org/10.1016/S0006-3495(03)74577-9 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Electric Vehicle Charging Route Planning for Shortest Travel Time Based on Improved Ant Colony Optimization.
Sensors (Basel)
December 2024
School of Cyber Science and Engineering, Liaoning University, Shenyang 110036, China.
Electric vehicles (EVs) are gaining significant attention as an environmentally friendly transportation solution. However, limitations in battery technology continue to restrict EV range and charging speed, resulting in range anxiety, which hampers widespread adoption. While there has been increasing research on EV route optimization, personalized path planning that caters to individual user needs remains underexplored.
View Article and Find Full Text PDFScaling Behavior of Ionic Conductance Dependent on Surface Charge Inside a Single-Digit Nanopore.
Molecules
January 2025
School of Mechanical Engineering, Chongqing Three Gorges University, Chongqing 404100, China.
Article Synopsis
- The ionic conductance in charged nanopores exhibits a power-law behavior at low salinity, influenced by surface charges that affect zeta potential and ion distributions.
- Accurately measuring surface charge density in single-digit nanopores poses challenges, leading researchers to develop new methodologies to investigate these effects.
- Through experiments with silicon nitride nanopores, a modified conductance model was established to analyze the relationship between pore concentration, surface charges, and potential leakage, allowing for a better understanding of ion mobility in nanopores.
Correspondence between Euler charges and nodal-line topology in Euler semimetals.
Sci Adv
January 2025
New Cornerstone Science Laboratory, Department of Physics, The University of Hong Kong, Hong Kong 999077, China.
Real multi-bandgap systems have non-abelian topological charges, with Euler semimetals being a prominent example characterized by real triple degeneracies (RTDs) in momentum space. These RTDs serve as "Weyl points" for real topological phases. Despite theoretical interest, experimental observations of RTDs have been lacking, and studies mainly focus on individual RTDs.
View Article and Find Full Text PDFImproved Mechanical Performances of Hastelloy C276 Composite Coatings Reinforced with SiC by Laser Cladding.
Nanomaterials (Basel)
December 2024
School of Mechanical and Electrical Engineering, Soochow University, Suzhou 215137, China.
Composite coatings reinforced with varying mass fractions of SiC particles were successfully fabricated on 316 stainless steel substrates via laser cladding. The phase compositions, elemental distribution, microstructural characteristics, hardness, wear resistance and corrosion resistance of the composite coatings were analyzed using X-ray diffraction (XRD), scanning electron microscopy (SEM), energy-dispersive X-ray spectroscopy (EDS), Vickers hardness testing, friction-wear testing and electrochemical methods. The coatings have no obvious pores, cracks or other defects.
View Article and Find Full Text PDFVisual Location of Oxygen Vacancies on Bismuth Titanate Nanosheets with Periodic Quantum Well and Promoting HO Photosynthesis.
Small
January 2025
Shandong Provincial Key Laboratory of Molecular Engineering, School of Chemistry and Chemical Engineering, Qilu University of Technology (Shandong Academy of Sciences), Jinan, Shandong, 250353, P. R. China.
Oxygen vacancy (OV) defect engineering plays a crucial role in enhancing photocatalytic efficiency. However, the direct visual characterization of oxygen vacancies still remains technically limited. Herein, a bismuth titanate (BiTiO, BTO-OV) model photocatalyst containing oxygen vacancies is constructed through density functional theory (DFT) calculations to reveal the influence mechanism of distinctive periodic quantum well and oxygen vacancies on the charge transfer behavior in BTO.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!