To analyze whether prolactin (PRL) in human follicular fluid (FF) is synthesized locally or derived from the circulation, PRL concentrations of plasma and FF were determined in the patients after ovarian stimulations. The amounts of PRL messenger ribonucleic acid (mRNA) in the follicular tissues during different menstrual phases were also determined. The FF PRL concentration was correlated positively with plasma PRL and highest estradiol levels during the stimulatory cycle. No PRL mRNA sequence was detected in the RNAs extracted from follicles at any stage in the menstrual cycle, although beta-actin mRNA was detected in all samples. In a comparison with pituitary RNA, the PRL mRNA concentration in ovarian follicular tissues seemed to be 10,000 times less than that in the pituitary. These results suggest that FF PRL may not be synthesized locally, but derived from the pituitary via the circulation through passive diffusion, and thus regulated by estrogen.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1507/endocrj1954.39.601 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
The CREBBP lysine acetyltransferase (KAT) is frequently mutated in follicular lymphoma (FL) and diffuse large B-cell lymphoma (DLBCL) and has been studied using gene knock-out (KO) in murine and human cells. However, the majority of CREBBP mutations encode amino acid substitutions within the catalytic KAT domain (CREBBP KAT-PM) that retain an inactive protein and have not been extensively characterized. Using CRISPR gene editing and extensive epigenomic characterization of lymphoma cell-lines, we found that CREBBP KAT-PM lead to unloading of CREBBP from chromatin, loss of enhancer acetylation and prevention of EP300 compensation.
View Article and Find Full Text PDFNanomicelle curcumin improves oxidative stress, inflammatory markers, and assisted reproductive techniques outcomes in endometriosis cases: a randomized clinical trial.
Naunyn Schmiedebergs Arch Pharmacol
March 2025
Department of Reproductive Biology, Academic Center for Education, Culture, and Research (ACECR), Qom, Iran.
We studied the effect nanomicelle curcumin on oxidative stress markers, pro-inflammatory cytokines, and assisted reproductive techniques (ART) outcomes in endometriosis cases. This randomized controlled trial was conducted on 50 women with endometriosis at the age of 25-35 candidate for ART referred to Roya Infertility Center in Qom, Iran, 2022. The participants were allocated to intervention (n = 25) and control (n = 25) groups by the blocked randomization method.
View Article and Find Full Text PDFEvaluation of the effect of human testicular cell conditioned media on the in vitro development of follicles from cryopreserved human ovarian cortical pieces. A potential approach for fertility preservation for cancer patients.
Cryobiology
March 2025
Molecular Medicine Research Center, Research Institute of Basic Medical Sciences, Rafsanjan University of Medical Sciences, Rafsanjan, Iran. Electronic address:
If in vitro culture conditions could be improved, in vitro growth of cryopreserved ovarian tissue and isolated follicles could become an alternative to ovarian tissue transplantation. This study evaluated two cryopreservation methods, slow cooling and vitrification, and two in vitro culture methods, to determine their effect on the in vitro growth of human ovarian follicles. After warming, the OT pieces (1 × 10 × 3 mm) were cultured in either routine culture medium (DMEM), or DMEM supplemented 50:50 with media previously used for human testicular cell culture (hTCCM) to serve as a source of growth factors.
View Article and Find Full Text PDFDevelopment and validation of a HPLC-MS/MS method the determination of genistein and equol in serum, urine and follicular fluid.
J Pharm Biomed Anal
March 2025
Department of Reproductive Endocrinology, Zhejiang Provincial People's Hospital, Hangzhou Medical College, Hangzhou, China. Electronic address:
Soy isoflavones exert estrogen-like synergistic or antagonistic effects by binding to estrogen receptors, and potentially impact the function of female reproductive system, but their distribution profile in human remains little clarified. To determination of genistein (GEN) and equol (EQ) in human urine, serum and follicular fluid (FF), an analytical method based on high-performance liquid chromatography-tandem mass spectrometry (HPLC-MS/MS) was developed and validated. The enrichment and clean-up are performed on a solid-phase extraction (SPE) column; the elution is a gradient one, with the mobile phase (A) of 0.
View Article and Find Full Text PDFFVB but Not B6 Mice Carrying the Thr92Ala-Dio2 Polymorphism Have Impaired Thyroid Hormonogenesis and Goiter.
Endocrinology
March 2025
Department of Internal Medicine, University of Texas Medical Branch, Galveston, USA.
The Thr92Ala-Dio2 polymorphism is prevalent worldwide, with about 50% of the population carrying at least one allele. The Ala92-Dio2 allele encodes a less active D2 enzyme and has been associated with neurodegenerative diseases, hypertension, and insulin resistance. To understand why its phenotypic effects are variable across different populations, in this study, we examined the impact of genetic background on the Thr92Ala-Dio2 polymorphism.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!