We developed a reliable and efficient, fully automated method for high-throughput isolation of genomic DNA from buccal swabs, based on silica-membrane technology. It was demonstrated that DNA purified using this method performed successfully in downstream applications relevant to forensic science and molecular diagnostics.
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http://dx.doi.org/10.1016/s1344-6223(02)00099-8 | DOI Listing |
Int J Syst Evol Microbiol
January 2025
Department of Health Technology and Informatics, The Hong Kong Polytechnic University, Hong Kong Special Administrative Region, Hong Kong, PR China.
A clinical isolate, R131, was isolated from the peritoneal swab of a patient who suffered from ruptured appendicitis with abscess and gangrene in Hong Kong in 2018. Cells are facultatively anaerobic, non-motile, Gram-positive coccobacilli. Colonies were small, grey, semi-translucent, low convex and alpha-haemolytic.
View Article and Find Full Text PDFMicrobiol Resour Announc
January 2025
Industrial Genomics Laboratory, FEMSA Biotechnolgy Center, School of Engineering and Sciences, Tecnológico de Monterrey, Monterrey, México.
sp. CC302I was isolated from a highly oligothrophic environment. High-throughput screening shows high codon-readthrough activity for the isolate with no canonical biosynthetic gene cluster responsible.
View Article and Find Full Text PDFFoodborne Pathog Dis
January 2025
Center for Disease Control and Prevention of Sichuan Province, Chengdu, China.
In recent years, infection is a major global public health concern, particularly in food safety. This study analyzed the genomes of 102 strains isolated between 2016 and 2023 from food, foodborne disease patients, and food poisoning incidents, focusing on their molecular characteristics, antibiotic resistance genes (ARGs), and virulence genes. serovar Enteritidis (37.
View Article and Find Full Text PDFMicrobiol Resour Announc
January 2025
Department of Natural Sciences, Northwest Missouri State University, Maryville, Missouri, USA.
Soil bacteria were isolated from the Tar Creek Superfund site in the presence of cadmium. Eight of these strains belong to the genus , whereas one strain belongs to the genus . Here, we report drafts of their genome sequences and highlight cadmium-resistance genes required in an exceptionally contaminated environment.
View Article and Find Full Text PDFArthritis Rheumatol
January 2025
Department of Biology and Biotechnologies "Charles Darwin", Sapienza University of Rome, Rome, Italy.
Objective: A pathogenetic role of CD8+ T lymphocytes in radiographic axial spondyloarthritis (r-axSpA) and other spondyloarthritis (SpA) is sustained by genome-wide association studies (GWAS) and by the expansion of public T cell clonotypes in the target tissues. This study investigates the migration of CD8+ T cells, along with their phenotype and functions in patients with r-axSpA and psoriatic arthritis (PsA).
Methods: Peripheral blood CD8+ and CD4+ T cells were isolated from r-axSpA (n= 128), PsA (n= 60) and rheumatoid arthritis (RA, n= 74) patients and healthy donors (HD, n= 79).
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