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Cell Death Mechanisms in a Mouse Model of Retinal Degeneration in Spinocerebellar Ataxia 7.
Neuroscience
February 2019
Inserm U1138. Centre des Recherches des Cordeliers, 15, rue de l'Ecole de Médecine, 78006 Paris, France; Université Pierre et Marie Curie, France; Université Paris Descartes, France. Electronic address:
Spino-cerebellar ataxia type 7 (SCA7) is a polyglutamine (polyQ) disorder characterized by neurodegeneration of the brain, cerebellum, and retina caused by a polyglutamine expansion in ataxin7. The presence of an expanded polyQ tract in a mutant protein is known to induce protein aggregation, cellular stress, toxicity, and finally cell death. However, the consequences of the presence of mutant ataxin7 in the retina and the mechanisms underlying photoreceptor degeneration remain poorly understood.
View Article and Find Full Text PDFThe hidden side of SERPINB1/Leukocyte Elastase Inhibitor.
Semin Cell Dev Biol
February 2017
INSERM U1138, Centre de Recherches des Cordeliers, Université Paris Descartes, Université Pierre et Marie Curie, Paris, France; Ecole Nationale Vétérinaire d'Alfort, France.
SERPINB1, also called Leukocyte Elastase Inhibitor (LEI) is a member of the clade B of SERPINS. It is an intracellular protein and acts primarily to protect the cell from proteases released into the cytoplasm during stress. Its role in inflammation is clear due to its involvement in the resolution of chronic inflammatory lung and bowel diseases.
View Article and Find Full Text PDFDNase I aggravates islet β-cell apoptosis in type 2 diabetes.
Mol Med Rep
June 2016
Department of Pharmacy, Beijing Tiantan Hospital, Capital Medical University, Beijing 100050, P.R. China.
Deoxyribonuclease I (DNase I) is an endonuclease responsible for the destruction of chromatin during apoptosis. However, its role in diabetes remains unclear. The aim of the current study was to investigate the role of DNase I combined with high glucose levels in β‑cell apoptosis.
View Article and Find Full Text PDFIncrease in the expression of leukocyte elastase inhibitor during wound healing in corneal endothelial cells.
Cell Tissue Res
December 2015
Departamento de Bioquímica, Facultad de Medicina, Universidad de la República, Gral. Flores 2125, 11800, Montevideo, Uruguay.
Tissue injury triggers a complex network of cellular and molecular responses. Although cell migration and proliferation are the most conspicuous, several other responses, such as apoptosis and increased protease activity, are necessary for a proper restitution of the tissue. In this work, we study the leukocyte elastase inhibitor (LEI) expression during wound healing of bovine corneal endothelial monolayers in culture.
View Article and Find Full Text PDFOn the use of an appropriate TdT-mediated dUTP-biotin nick end labeling assay to identify apoptotic cells.
Anal Biochem
July 2015
Centre de Recherches des Cordeliers, INSERM U1138, Université Pierre et Marie Curie, Université Paris Descartes, 75006 Paris, France. Electronic address:
Article Synopsis
- Apoptosis is a crucial process for development and tissue health, marked by DNA fragmentation through enzymes like caspase-activated DNase (CAD).
- The TUNEL technique is commonly used to identify apoptotic cells by labeling specific DNA ends, but it only works well for caspase-dependent apoptosis and not for other pathways that result in different types of DNA ends.
- The proposed modification to the TUNEL protocol involves adding a dephosphorylation step, enabling the detection of both caspase-dependent and independent DNA breaks, which improves the accuracy of measuring cell death in various treatments.
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