A selective interaction of ATA with RNA, unlike DNA, when isolating nucleic acids from plant materials was established. The data concerning the binding strength of highly purified RNAATA and DNAATA preparations to nitrocellulose and nylon filters under conditions of high ionic strength are presented. The interrelation of ATA RNA-tropism and absence of adsorption capability of chemical modified RNAATA the backbone was observed. Using IR spectroscopy under the procedure of multi-broken complete light reflection, a formation of ATA and RNA complex was fixed via phosphoric-ether bond (P-O-C), which was absent in the case of DNAATA. The obtained data raise the problem of RNAATA application in molecular biology experiments.
Download full-text PDF |
Source |
|---|
Publication Analysis
Top Keywords
Similar Publications
Objective: Scleroderma-associated autoantibodies (SSc-Abs) are specific in participants (pts) with systemic sclerosis and are associated with organ involvement. Our objective was to assess the influence of baseline SSc-Abs on the trajectories of the clinical outcome assessments (COAs) in a phase III randomized controlled trial.
Methods: We used data on both the groups who received placebo (Pbo) and tocilizumab from the focuSSced trial.
Analysis of IL10 gene promoter haplotypes and changes in mRNA expression and soluble levels in patients with basal cell carcinoma.
Arch Dermatol Res
January 2025
Instituto de Investigación en Ciencias Biomédicas (IICB), Centro Universitario de Ciencias de La Salud, Universidad de Guadalajara, 44340, Guadalajara, Mexico.
Interleukin-10 (IL-10) is an immunomodulatory molecule that may play an immunosuppressive role in nonmelanoma skin cancer (NMSC), specifically basal cell carcinoma (BCC). We analyzed the role of IL10 promoter variants in genetic determinants of BCC susceptibility and their association with IL10 mRNA and IL-10 serum levels. Three promoter variants (- 1082 A > G, - 819 T > C, and - 592 A > C) were examined in 250 BCC patients and 250 reference group (RG) individuals.
View Article and Find Full Text PDFCharacterization of the Mitochondrial Genome of (Astacidea: Cambaridae) and Its Phylogenetic Implications.
Genes (Basel)
December 2024
Key Laboratory of Prevention and Control of Zoonotic Diseases of Daqing, College of Animal Science and Veterinary Medicine, Heilongjiang Bayi Agricultural University, Daqing 163319, China.
Background: is an endangered freshwater crayfish in China, belonging to the genus Cambaroides, that can act as a complementary host for paragonimus. The objective of this study was to examine the complete mitochondrial genome characteristics and their evolutionary relationships within the Astacidea.
Methods: The analysis of gene rearrangements and evolutionary relationships was conducted through the sequencing of the mitochondrial genome of .
Impact of autoantibody status on stratifying the risk of organ involvement and mortality in SSc: experience from a multicentre French cohort of 1605 patients.
RMD Open
November 2024
Department of Internal Medicine, Infectious Diseases, and Clinical Immunology, University Hospital Centre Reims, Reims, Grand Est, France.
Article Synopsis
- Systemic sclerosis (SSc) is a rare autoimmune disease classified into two groups based on skin involvement; this study investigates the role of autoantibody profiles in predicting organ damage and mortality compared to skin phenotype.
- Researchers analyzed data from 1,605 patients across seven French hospitals, focusing on autoantibodies and their correlation with various organ issues and death rates.
- Findings reveal that specific autoantibodies are strongly linked to serious complications like interstitial lung disease and renal crisis, while skin phenotype alone does not effectively predict patient outcomes.
The Full-Length Genomic Sequence of the HLA-B*39:130 Allele.
HLA
November 2024
Laboratory of Cellular and Molecular Biology, College of Basic Medical Sciences, Central South University, Changsha, Hunan, China.
HLA-B*39:130 differs from HLA-B*39:01:01:01 at codon 214 (ACA → ATA) and position 355 (T → C) in intron 5.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!