Human and murine antigens are purified by exclusion chromatography on Sephadex and preparative polyacrylamide gel electrophoresis and their purification stage checked by analytical methods. The study of antigenic preparations consists of electrofocusing, molecular weight estimation and chemical determinations. A cytotoxicity inhibition test with specific alloantisera reveals the antigenic potency. A microtest using 51Cr labelled tumoral cells has been achieved for the analysis of murine preparations.
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