Objective: To observe the effect of adrenomedullin (ADM) on the production of nitric oxide (NO) in rat aorta and the effect of Proadrenomedullin N-terminal 20 peptide (PAMP) and adrenotensin (ADT) on the ADM-induced NO production.
Methods: Isolated aortic tissues were exposed to ADM, PAMP and ADT for 2 h. The NO production, indicated by nitrite content in the incubated media, and the nitric oxide synthase (NOS) activity in the incubated tissues were assayed.
Results: Nitrite productions and NOS activities of the aortic tissues were significantly increased by ADM in a concentration-dependent manner. The nitrite production and NOS activity of the aortic tissues stimulated by ADM (10(-8) mol.L-1) incubation were (0.282 +/- 0.046) mumol per mg protein and (0.323 +/- 0.056) pmol.min-1 per mg protein, respectively, which were greater than those of the control (0.173 +/- 0.026) mumol per mg protein and (0.110 +/- 0.028) pmol.min-1 per mg protein (P < 0.01), respectively. The nitrite production and NOS activity were (0.204 +/- 0.049) mumol per mg protein and(0.178 +/- 0.023) pmol.min-1 per mg protein when the tissues were treated with ADM (10(-8) mol.L-1) and PAMP (10(-8) mol.L-1) in combination, and were (0.150 +/- 0.036) mumol per mg protein and (0.123 +/- 0.031) pmol.min-1 per mg protein when ADM (10(-8) mol.L-1) and ADT (10(-8) mol.L-1) were used in combination, which were significantly less than those in ADM (10(-8) mol.L-1) group. After incubation of the aortic tissues with the same concentrations(10(-8) mol.L-1) of ADM, PAMP and ADT in combination, the nitrite production and NOS activity were (0.162 +/- 0.029) mumol per mg protein and (0.110 +/- 0.024) pmol.min-1 per mg protein, which were also greatly reduced as compared with those of the ADM group (10(-8) mol.L-1, P < 0.01). However, neither PAMP nor ADT had effect on the production of nitrite and NOS activity in the aortic tissues.
Conclusion: ADM enhanced the NO production in rat aorta, which was antagonized by PAMP and ADT alone or in combination through influencing the NOS activity.
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