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Underlying mechanisms preserving coronary basal tone and NO-mediated relaxation in obesity: Involvement of β1 subunit-mediated upregulation of BK channels.
Atherosclerosis
August 2017
Departamento de Fisiología, Facultad de Farmacia, Universidad Complutense, Madrid, Spain.
Background And Aims: The impact of obesity on vasomotor regulation of coronary arteries and its underlying mechanisms are not completely understood and, in particular, the role of BK channels in the NO-mediated coronary vasodilation in obesity remains to be elucidated.
Methods: The effects of selective blockade of BK channel was tested on nitric oxide (NO)-mediated vasodilator responses of coronary arteries from lean and obese Zucker rats (LZR and OZR, respectively) by means of simultaneous measurements of intracellular Ca concentration ([Ca]) by Fura-2 fluorescence and tension in endothelium-denuded coronary arteries mounted in microvascular myographs. BK channel subunits expression was measured by Western blot.
Contribution of endoplasmic reticulum Ca2+ regulatory mechanisms to the inflammation-induced increase in the evoked Ca2+ transient in rat cutaneous dorsal root ganglion neurons.
Cell Calcium
July 2013
Center for Pain Research, University of Pittsburgh, Pittsburgh, PA 15213, USA.
Persistent inflammation results in an increase in the magnitude and duration of high K(+)-evoked Ca(2+) transients in putative nociceptive cutaneous dorsal root ganglion (DRG) neurons. The purpose of the present study was to determine whether recruitment of Ca(2+)-induced Ca(2+) release (CICR) contributes to these inflammation-induced changes. Acutely dissociated, retrogradely labeled cutaneous DRG neurons from naïve and complete Freund's adjuvant inflamed adult male Sprague-Dawley rats were studied with ratiometric microfluorimetry.
View Article and Find Full Text PDFRole of stored calcium in the regulation of neurotransmitter quantum size.
Bull Exp Biol Med
February 2012
Department of Human and Animal Physiology, Biological Faculty, M. V. Lomonosov Moscow State University, Moscow, Russia.
Release of stored calcium ions during activation of ryanodine receptors with ryanodine or caffeine elevates the mean amplitude of spontaneous miniature end-plate potentials. Blockade of these receptors with selective antagonists abolishes this effect. Preliminary loading of the motor nerve terminals with intracellular calcium buffer EGTA-AM, but not with BAPTA-AM, also completely prevented the ryanodine-induced increment of miniature end-plate potential amplitude probably induced by the release of stored calcium.
View Article and Find Full Text PDFMutational analysis of putative calcium binding motifs within the skeletal ryanodine receptor isoform, RyR1.
J Biol Chem
December 2004
Department of Anesthesia Research, Brigham and Women's Hospital, Boston, MA 02115, USA.
The functional relevance of putative Ca(2+) binding motifs previously identified with Ca(2+) overlay binding analysis within the skeletal muscle ryanodine receptor isoform (RyR1) was examined using mutational analysis. EF hands between amino acid positions 4081 and 4092 (EF1) and 4116 and 4127 (EF2) were scrambled singly or in combination within the full-length rabbit RyR1 cDNA. These cDNAs were expressed in 1B5 RyR-deficient myotubes and channel function assessed using Ca(2+)-imaging techniques, [(3)H]ryanodine binding measurements, and single channel experiments.
View Article and Find Full Text PDFCaffeine- and ryanodine-induced changes in the spectrum of spontaneously secreted quanta of the mediator in the neuromuscular synapse of mice.
Dokl Biol Sci
October 2003
Biological Faculty, Moscow State University, Vorob'evy gory, Moscow, 119899 Russia.
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