We have established a simple diagnosis method for rice blast fungus resistant to MBI-D. This involves the preparation of PCR templates directly from the lesions in combination with primer-introduced restriction enzyme analysis PCR (PIRA-PCR).
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Genetic variation of as a protective genotype for the development of colorectal cancer in men.
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Background: The role of transforming growth factor beta (TGF-β) signaling, including both the cytokine and their receptors, in the etiology of colorectal cancer (CRC) has been of particular interest lately.
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Novel genotyping assay for the gene mutation and its allele frequency in Border Collie dogs in Mexico.
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Departamento de Producción Animal, División de Ciencias Veterinarias, Centro Universitario de Ciencias Biológicas y Agropecuarias, Universidad de Guadalajara, Jalisco, México.
A 4-bp deletion in the ATP-binding cassette subfamily B member 1 () gene, also referred to as the multidrug resistance gene (), produces stop codons that cause premature termination of P-glycoprotein 1 (P-gp) synthesis. Dogs with the homozygous mutation do not express functional P-gp, which increases their sensitivity markedly to many common veterinary drugs. We detected the mutation in Border Collie dogs in western Mexico with a simple and affordable primer-introduced restriction analysis PCR (PIRA-PCR).
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Animal genetics & Breeding Division, ICAR-Central Institute for Research on Cattle, Meerut, UP, India.
Background: DNA testing in the cattle industry undergoes multiple hurdles. Successful genotyping involves the transportation of samples from the field to the laboratory in a chilled environment followed by DNA extraction, and finally, a specific genotyping protocol is followed. Various researches are focused on overcoming these issues.
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Iran J Vet Res
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Animal Genetics and Breeding Section, ICAR-Central Institute for Research on Cattle, Meerut Cantt, Meerut, 250001, Uttar Pradesh, India.
Complex vertebral malformation (CVM) has considerable economic impact on dairy cattle breeding due to extensive use of artificial insemination (AI). Identifying the carrier is an important factor to reduce the incidence of the genetic disorder. The study was conducted to identify the carriers of CVM in Frieswal cattle by polymerase chain reaction-primer-introduced restriction analysis (PCR-PIRA) method, which was further confirmed by sequencing.
View Article and Find Full Text PDFComparison of allele-specific PCR, created restriction-site PCR, and PCR with primer-introduced restriction analysis methods used for screening complex vertebral malformation carriers in Holstein cattle.
J Vet Sci
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Department of Animal Breeding and Husbandry, Faculty of Veterinary Medicine, Istanbul University, Istanbul 34320, Turkey.
Complex vertebral malformation (CVM) is an inherited, autosomal recessive disorder of Holstein cattle. The aim of this study was to compare sensitivity, specificity, positive and negative predictive values, accuracy, and rapidity of allele-specific polymerase chain reaction (AS-PCR), created restriction-site PCR (CRS-PCR), and PCR with primer-introduced restriction analysis (PCR-PIRA), three methods used in identification of CVM carriers in a Holstein cattle population. In order to screen for the G＞T mutation in the solute carrier family 35 member A3 () gene, DNA sequencing as the gold standard method was used.
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