Co-expression in Escherichia coli of wild-type (wt) hepatitis B virus core protein (HBc) and its naturally occurring variants with deletions at amino acid positions 77-93 or 86-93 leads to formation of mosaic particles, which consist of three dimer subunit compositions. These compositions are wt/variant HBc heterodimers and two types of homodimers, formed by wt HBc or the variant HBc themselves. Mosaic particles were found also when both HBc deletion variants 77-93 and 86-93 were co-expressed in E. coli. These findings are discussed in terms of their significance for hepatitis B virus pathogenesis and prospective use of mosaic particles in vaccine development.
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http://dx.doi.org/10.1016/s0014-5793(03)00805-6 | DOI Listing |
Nat Rev Bioeng
November 2024
Aiiso Yufeng Li Family Department of Chemical and Nano Engineering, University of California, San Diego, La Jolla, CA, USA.
Viruses can be designed to be tools and carrier vehicles for intratumoural immunotherapy. Their nanometre-scale size and shape allow for functionalization with or encapsulation of medical cargoes and tissue-specific ligands. Importantly, immunotherapies may particularly benefit from the inherent immunomodulatory properties of viruses.
View Article and Find Full Text PDFSmall Sci
July 2024
Department of NanoEngineering, University of California San Diego, 9500 Gilman Dr., La Jolla, California 92093, United States.
J Agric Food Chem
December 2024
State Key Laboratory of Green Pesticides, Key Laboratory of Green Pesticide and Agricultural Bioengineering, Ministry of Education, Center for R&D of Fine Chemicals of Guizhou University, Guiyang 550025, China.
Viral nanoparticles (VNPs) are self-assembled nanometric complexes whose size and shape are similar to those of the virus from which they are derived. VNPs are arousing great attention due to potential biotechnological applications in fields like nanomedicine and nanotechnology because they allow the presentation of polypeptides of choice linked to the virus structural proteins. Starting from tobacco etch virus (TEV), a plant plus-strand RNA virus that belongs to the genus (family ), here we describe the development of recombinant hybrid VNPs in plants able of exposing simultaneously different proteins on their surface.
View Article and Find Full Text PDFJ Biomol Struct Dyn
November 2024
Department of Chemistry, Faculty of Science, Yazd University, Yazd, Iran.
The Tobacco Mosaic Virus (TMV) is a critical plant virus that can cause a significant drop in crop yield. To understand how recombinant coat-protein impacts the affinity and assembly of TMV's subunits, research is being conducted to assess the effect of recombinant protein on virus resistance. To develop a recombinant coat-protein that can lower TMV infection rates in plants, a design strategy was employed that involves creating defective viral subunits leading to incorrect assembly.
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