AI Article Synopsis

  • Co-expression of wild-type hepatitis B virus core protein and its deletion variants in E. coli results in mosaic particles composed of various dimer subunit combinations.
  • These combinations include heterodimers of the wild-type and variant proteins, as well as homodimers formed from either the wild-type or variant proteins alone.
  • The study's findings have implications for understanding hepatitis B virus pathogenesis and potential applications in vaccine development.

Article Abstract

Co-expression in Escherichia coli of wild-type (wt) hepatitis B virus core protein (HBc) and its naturally occurring variants with deletions at amino acid positions 77-93 or 86-93 leads to formation of mosaic particles, which consist of three dimer subunit compositions. These compositions are wt/variant HBc heterodimers and two types of homodimers, formed by wt HBc or the variant HBc themselves. Mosaic particles were found also when both HBc deletion variants 77-93 and 86-93 were co-expressed in E. coli. These findings are discussed in terms of their significance for hepatitis B virus pathogenesis and prospective use of mosaic particles in vaccine development.

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http://dx.doi.org/10.1016/s0014-5793(03)00805-6DOI Listing

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