Aim: To establish the whole rDNA ITS region sequence database of various Dendrobium species of "Fengdou" and to authenticate exactly the inspected species of "Fengdou".
Methods: The rDNA ITS regions of various Dendrobium species of "Fengdou" were amplified and sequenced. The database of their rDNA ITS regions was established in order to authenticate the inspected species by means of the softwares of CLUSTRAL and MEGA which were used to analyze the rDNA ITS region.
Results: A database of the rDNA ITS sequences of 21 species of Dendrobium has been established. The notable and stable differences of the interspecies of the rDNA ITS regions have been demonstrated. The numbers of transitions and transversions among 21 species are 11-122. The variable sites are 341 while the informative sites are 195. The ITS sequence differences between the outgroup species (Pholidota yunnanensis) and species of "Fengdou" are obvious. The numbers of transitions and transversions are 131-161. The population differences of the rDNA ITS region of various species of "Fengdou" are very small (0-6).
Conclusion: On the basis of the database of various Dendrobium species of "Fengdou" and two genetics software, the botanical origin of the inspected species of "Fengdou" has been authenticated successfully by sequencing the rDNA ITS regions.
Download full-text PDF |
Source |
|---|
Publication Analysis
Top Keywords
Similar Publications
Integrated Analysis of Metabolomics Combined with Network Pharmacology and Molecular Docking Reveals the Effects of Processing on Metabolites of .
Metabolites
July 2023
Key Laboratory of Tropical Fruits and Vegetables Quality and Safety for State Market Regulation, School of Food Science and Engineering, Hainan University, Haikou 570228, China.
() is a precious medicinal species of Orchidaceae, and the product obtained by hot processing is called "Fengdou". At present, the research on the processing quality of mainly focuses on the chemical composition indicators such as polysaccharides and flavonoids content. However, the changes in metabolites during processing are still unclear.
View Article and Find Full Text PDFDevelopment, Identification, and Application of a Germplasm Specific SCAR Marker for Kimura et Migo.
Front Plant Sci
May 2021
College of Life and Environmental Sciences, Hangzhou Normal University, Hangzhou, China.
The stems of have been used as a rare and valuable Chinese tonic medicine, known as "Tiepi Fengdou", since the Qing dynasty. Because of the increased market demand and continued exploitation of this plant, the reserves of wild resources have been depleted, and products on the market are being increasingly adulterated. Such changes have strongly affected the sustainable utilization of this valuable medicinal plant resource and the development of related industries.
View Article and Find Full Text PDFThe large single-copy (LSC) region functions as a highly effective and efficient molecular marker for accurate authentication of medicinal species.
Acta Pharm Sin B
October 2020
College of Life Sciences, Nanjing Normal University, Nanjing 210023, China.
Having great medicinal values, species of "Fengdou" (DSFs) are a taxonomically complex group in genus including many closely related and recently diverged species. Traditionally used DNA markers have been proved to be insufficient in authenticating many species of this group. Here, we investigated 101 complete plastomes from 23 DSFs, comprising 72 newly sequenced and 29 documented, which all exhibited well-conserved genomic organization and gene order.
View Article and Find Full Text PDFPlastome-wide comparison reveals new SNV resources for the authentication of and its corresponding medicinal slice (Huoshan Fengdou).
Acta Pharm Sin B
May 2018
College of Life Sciences, Nanjing Normal University, Nanjing 210023, China.
species and their corresponding medicinal slices have been extensively used as traditional Chinese medicine (TCM) in many Asian countries. However, it is extremely difficult to identify species based on their morphological and chemical features. In this study, the plastomes of were used as a model system to investigate the hypothesis that plastomic mutational hotspot regions could provide a useful single nucleotide variants (SNVs) resource for authentication studies.
View Article and Find Full Text PDFExtraction and Analysis of Gigantol from Dendrobium officinale with Response Surface Methodology.
Molecules
April 2018
College of Life Science, Anhui Agricultural University, Hefei 230036, China.
In order to optimize the extraction of gigantol from , the influence of methanol concentration, ultrasonic temperature, and liquid ratio on extraction efficiency was analysed by the response surface analysis method. The results show that the extraction rate reached a maximum when the methanol concentration was 92.98%, the solid-liquid ratio was 27.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!