AI Article Synopsis
Article Abstract
Diagnostic potential of the Chlamydia trachomatis ligase chain reaction system (LCx) to assess the presence of C. trachomatis in urine and semen specimens was evaluated. Paired urine and semen specimens from 153 asymptomatic male partners of subfertile couples attending our Center for Reproductive Medicine were examined by LCx. As controls, 19 semen samples from four donors who were participating in the programme for artificial insemination were used. Of these, 12 samples had previously been shown to be C. trachomatis-positive by an in-house PCR. C. trachomatis was detected by LCx in seven of 153 (5 %) urine samples. None of the 153 semen samples tested positive by LCx. Also, none of the 12 C. trachomatis-containing control semen samples were positive by LCx. By in-house PCR, seven urine specimens and two of 153 (1 %) semen samples tested positive. The corresponding urine samples of these male partners were also C. trachomatis-positive, as well as the 12 C. trachomatis-containing samples from donors. In conclusion, LCx is not sensitive enough to assess the presence of C. trachomatis in semen specimens; therefore, this method is not recommended to routinely screen semen specimens from donors who participate in programmes for artificial insemination or male partners of subfertile couples for C. trachomatis.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1099/jmm.0.05187-0 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Validation of a mail-in delayed semen analysis protocol developed for home collection.
F S Rep
December 2024
Quest Diagnostics, Secaucus, New Jersey.
Objective: To validate a mail-in delayed semen analysis service using deidentified remnant samples from a US fertility clinic.
Design: Double-blinded prospective validation of screening/diagnostic test.
Setting: Fertility clinic and clinical reference laboratory.
Per- and Polyfluoroalkyl Substances in Semen Associated with Repeated Measures of Semen Quality in Healthy Adult Men.
Environ Sci Technol
January 2025
College of Environment and Climate, Guangdong Key Laboratory of Environmental Pollution and Health, Jinan University, Guangzhou 510632, China.
Although epidemiological studies have explored the association between poly- and perfluoroalkyl substances (PFAS) concentrations and semen quality, existing findings are often inconsistent. Our work aimed to explore the association of PFAS in plasma and semen with repeated measures of semen quality parameters in healthy adults. Plasma was collected at the initial recruitment and semen was collected at least once within five predetermined intervals during an approximately 3-month period.
View Article and Find Full Text PDFProteomic Analysis of Biological Fluids.
Methods Mol Biol
December 2024
Department of Environmental Toxicology, The University of California, Davis, Davis, CA, USA.
Biological fluids are proteinaceous liquids or suspensions released through different body orifices or through penetration of the skin. These fluids are the result of multiple tissues and cell types and contain extensive, highly complex, and dynamic protein populations that reflect both the transcriptional program of the originating cells and a record of the individual's health status. Body fluids are readily accessible to clinicians and researchers, and as such proteomic analyses are an important component of clinical studies, fertility studies, oral health studies, and forensic investigations.
View Article and Find Full Text PDFUnravelling spermatogenesis in spotted wolffish: Insights from the ultrastructure of juvenile male testes to the cryopreservation of broodstock sperm.
Aquaculture
November 2024
Department of Genomics, Faculty of Biosciences and Aquaculture, Nord University, Bodø 8049, Norway.
The aim of this study was to deepen our understanding of the reproductive biology of male spotted wolffish () using two different experimental approaches involving juvenile and mature broodstock fish. The first approach consisted of a detailed histological examination of the testes to identify the onset of gonadal maturation and characterise the spermatogenic stages in two- and three-year-old juvenile specimens. Light microscopy analysis revealed clear differences between the age groups.
View Article and Find Full Text PDFMale reproductive biotechnologies in South American Camelids Part I: Semen collection, evaluation and handling.
Anim Reprod Sci
January 2025
Universidad de Buenos Aires, Facultad de Ciencias Veterinarias, Instituto de Investigación y Tecnología en Reproducción Animal (INITRA), Ciudad Autónoma de Buenos Aires, Buenos Aires, Argentina.
This review describes the first steps necessary to apply any reproductive biotechnology in South American camelids (SAC) semen or sperm: sample collection, evaluation and handling. In camelids, the length and position adopted for mating and the site of semen deposition have conditioned semen collection methods. The advantages and disadvantages of available collection methods are summarized.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!