Objective: To investigate the relationship between the expressive variety of apoptosis-related gene Bcl-2, Fas and the brain injury time during earlier period after cerebral contusion.
Methods: The morphologia feature of brain tissue was observed under microscopy and immunohistochemistry, image analyze technique was applied to detected the expression of Bcl-2 and Fas.
Results: Typical apoptotic cells were seen 8 hours after cerebral contusion. The expression of apoptosis-related gene was noted after 30 minutes and keep increase subsequently. The expression of Bcl-2 reached maximum level at 4 hours after brain trauma and then declined.
Conclusion: Detecting the expression of Bcl-2 and Fas would be of valuable in the distinguishment of promortem and postmortem injury, and the estimation of brain injury time during the earlier period after cerebral contusion.
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